Strategy for the generation of GAD65-tdTomato mice expressing the fluorescent protein tdTomato under control of the GAD65 promoter in GABAergic neurons. per Stefanie Besser (755096)

“…The transgene consists of the ORF of tdTomato, a SV40-PolyA site as well as a FRT-flanked neomycin resistance cassette (neo) and was inserted directly after the endogenous ATG using homologous recombination provoked by homology arms (HA) indicated. <b>C:</b> Representation of the modified BAC containing the GAD65-tdTomato transgene after removal of the neomycin resistance cassette using Flp recombination. …”

(A) Creation of a genetic rescue construct encoding fused with an N-terminal FLAG-tetracysteine tag (4C), per Jaroslaw Kasprowicz (90262)

“…(b) PCR product containing left (L) and right (R) homology arms, the 4C tag, and LoxP site (gray triangles) flanked marker. …”

Background Selection as Baseline for Nucleotide Variation across the <i>Drosophila</i> Genome per Josep M. Comeron (128554)

“…<div><p>The constant removal of deleterious mutations by natural selection causes a reduction in neutral diversity and efficacy of selection at genetically linked sites (a process called Background Selection, BGS). …”

Conditional Chr 13 <i>PRELI</i> gene Targeting. per Wenbin Ma (320902)

“…Also indicated are 5′ and 3′ arms of homology, LoxP sites, Flip recombination (Frt) sequences, and PGK-Neo and HSV-Tk selection cassettes; <b>III</b>: Floxed <i>PRELI</i> allele, after Frt-mediated removal of selection cassettes; <b>IV</b>: <i>PRELI</i> deficient allele after CD19-driven Cre recombinase (Cre) cleavage of <i>PRELI</i> exon II. …”

Metadata supporting data files in the published article: DNA Methylation Markers Predict Recurrence-free Interval in Triple- Negative Breast Cancer per MaryJo Fackler (8134182)

“…</p> <p><b> </b></p> <p><b>Study approval: </b>All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. …”

Endogenous nutrient stores correlate with the CPI-613 response <i>in vitro</i> and <i>in vivo</i>. per Moises O. Guardado Rivas (12837125)

“…Note that other cell death studies herein generally involve longer incubation times and, thus, stronger responses.…”

Tsg101 is required for the NiV-C enhancement of budding and efficient release of live NiV. per Arnold Park (237682)

“…Destabilization of DD-Tsg101 by removal of TMP abrogates the NiV-C enhancement of NiV-M budding. …”

Design of the cGAL gene trap strategy. per Haania Khan (20598473)

“…<p><b>(A)</b> Schematic of creating a cGAL gene trap (cGAL GT) allele via CRISPR/Cas9-mediated homology-directed repair using two homology arms (5’ and 3’ HAs). The grey rectangles, black lines, and light grey boxes represent exons, introns, and UTRs of the target gene <i>X</i>, respectively. …”

The Isala Sisterhood per Sarah Lebeer (181744)

“…One way to set a societally relevant research agenda is actively involving citizens in scientific dialogues. We founded Isala as a citizen-science project to advance research, diagnostics, and therapeutics for the vaginal microbiome. …”

Components of the Ciona Tracker 2.0 and process of generating an agarose arena. per Athira Athira (13199796)

“…Black arrowhead indicates plastic arms that secure the PLA ring to the underlayer. …”

E06918: Aldhelm's poem On the Church of Mary Built by Bugga records the consecration on 15/16 August of a church with an apse dedicated to *Mary (Mother of Christ, S00033), constru... per bsavill (10125827)

“…<br><br>After him King Cædwalla, renowned in war and arms, succeeded to the kingdom, a powerful occupant of the throne and its rightful heir. …”

CENP-A^Cse4 mislocalization to promoters alters transcription. per Erica M. Hildebrand (3178719)

“…(E) Model: At chromosome arms, INO80-C-mediated full nucleosome turnover may lead to CENP-A^Cse4 deposition and H2A.Z^Htz1 removal. …”

iToBoS 2024 - Skin Lesion Detection with 3D-TBP per Anup Saha (20757917)

“…</p><p dir="ltr">The dataset provides diverse anatomical locations, including the torso, arms, and legs, with each image having an average resolution of 1012x827 pixels and a 45-pixel overlap between adjacent images. …”

Datasheet1_Randomized controlled phase IIa clinical trial of safety, pharmacokinetics and pharmacodynamics of tenofovir and tenofovir plus levonorgestrel releasing intravaginal rin... per Nelly R. Mugo (7901144)

“…Adverse events (AEs) were distributed similarly among the three arms. There were two non-product related AEs graded >2. …”

Periphery-Palladated Carbosilane Dendrimers:  Synthesis and Reactivity of Organopalladium(II) and -(IV) Dendritic Complexes. Crystal Structure of [PdMe(C₆H₄(O... per Neldes J. Hovestad (3025479)

“…These complexes can undergo subsequent reductive elimination at ambient temperature involving both Me−Ar and Me−CH₂Ph coupling on decomposition. …”

CAMBRIA-1 & CAMBRIA-2 phase III trials: camizestrant versus standard endocrine therapy in ER+/HER2– early breast cancer per Erika P. Hamilton (15004344)

“…CAMBRIA-2 is comparing 7 years of upfront adjuvant camizestrant versus endocrine therapy, with abemaciclib permitted in both treatment arms for the first 2 years. The primary endpoint for both studies is invasive breast cancer-free survival. …”

Totally Endoscopic Port-Access Artial Myxoma Resection Without Robotic Assistance per Nguyen Cong Huu (10906847)

“…The right side of the chest was slightly elevated at 300, two arms along the body. External defibrillator patches were placed to subtend the maximum cardiac mass <br> A 2 cm tranverse incision was made in the right groin and vessel cannulas<br> were established with indirect artery cannulation via a Dacron graft (8mm in diameter), anastomosed end-to-side to the right common femoral artery. …”

Preferential expression of <i>Mcmdc2</i> in the gonads, and <i>Mcmdc2</i> targeting in mice. per Friederike Finsterbusch (460563)

“…Recombination catalyzed by FLPe at FRT sites removes the SA-IRES-LacZ exon and the hBactP-Neo gene, and restores the MCMDC2 ORF (<i>Mcmdc2</i>^{<i>restored</i>}). …”

Construction and analysis of <i>lmp1</i> mutant <i>B. burgdorferi</i>. per Xiuli Yang (266545)

“…Viable spirochetes were incubated with (+) or without (−) proteinase K for the removal of protease-sensitive surface proteins and processed for immunoblot analysis using Lmp1 antibodies. …”

E06668: The 'epic' Greek Martyrdom of *Zenobios and Zenobia (brother and sister, martyrs of Aegae, S01681) recounts the miraculous accomplishments of the Christian healer Zenobios,... per Nikolaos (10112551)

“…When the doctors recommend surgical removal, she is afraid that she cannot withstand such pain. …”