Importance of nsp1 subdomains for transcription and virus production. de réir Danny D. Nedialkova (366971)

“…Total intracellular RNA was isolated at 11 h post-transfection and resolved by denaturing formaldehyde electrophoresis. …”

E4-ORF1 also assembles the ternary complex in adenovirus-infected cells. de réir Kathleen Kong (558240)

“…<p>(<b>A</b>) The ternary complex forms in cells infected with <i>wt</i> Ad9 virus but not with mutant Ad9-V125A virus that expresses PBM mutant V125A. …”

RAG-1 and Ly6D Independently Reflect Progression in the B Lymphoid Lineage de réir Qingzhao Zhang (241172)

“…However, it has been obvious for some time that CLPs are heterogeneous, and there has been controversy concerning their differentiation potential. We have now resolved four Flt3⁺ CLP subsets that are relatively homogenous and capable of forming B cells. …”

Individual-Based Model of Microbial Life on Hydrated Rough Soil Surfaces de réir Minsu Kim (716600)

“…This study presents a spatially-resolved model of microbial dynamics on idealised rough soil surfaces represented as patches with different (roughness) properties that preserve the salient hydration physics of real surfaces. …”

Table_1_Bullet-shaped magnetosomes and metagenomic-based magnetosome gene profiles in a deep-sea hydrothermal vent chimney.XLSX de réir Shinsaku Nakano (16454274)

“…Additionally, the electron microscopy of particles collected by magnetic separation from the chimney sample revealed MTB cells with bullet-shaped magnetosomes, and there were minor occurrences of cuboctahedral and hexagonal prismatic magnetosomes. Genome-resolved metagenomic analysis was performed to identify microorganisms that formed magnetosomes. …”

Northern analyses of <i>rrp47∆ rex1∆</i> and <i>rrp47∆ mpp6∆</i> mutants. de réir Monika Feigenbutz (479910)

“…<i>RRP6</i> constructs encoded either non-tagged or zz-tagged fusion proteins. RNA was resolved through 8% denaturing polyacrylamide gels and northern blot analyses performed, using probes complementary to the RNAs indicated on the right of each panel. …”

Supplement: Structure-based Hamiltonian model for IsiA uncovers a highly robust pigment protein complex de réir Hanan Schoffman (9237594)

“…Recently, the IsiA-PSI structure from <i>Synechocystis</i> sp. PCC 6803 was resolved to 3.48 Å. Based on this structure, we created a model simulating a single excitation event in an IsiA monomer. …”

Viability of yeast cells producing S100A8 and S100A9 on the background of a <i>ts</i> mutation in an essential gene. de réir Ekaterina Eremenko (386021)

“…After 2 days of induction ^GFPS100A9 forms aggregates in wild type and ts strain <i>cdc53-1</i> at 30°C and 32°C. …”

BN-PAGE analysis of mAb binding to point mutants of Env from HIV-1_JR-FL. de réir Johannes S. Gach (244457)

“…<p>MAbs (30 µg/mL) were incubated with JR-FL SOS gp160ΔCT VLPs, which were then gently solubilized with non-ionic detergent and resolved using BN-PAGE. Western blots were then probed with a cocktail of anti-Env mAbs. …”

Yeast complementation and thioester formation of VrUBC1. de réir Eunsook Chung (415821)

“…Reactions were resolved by SDS-PAGE and western blots were performed with with anti-His₆ antibodies.…”

<i>In vitro</i> characterization of SlmA variants. de réir Hongbaek Cho (282091)

“…Protein-DNA complexes were resolved on a 6% polyacrylamide gel and DNA was visualized using a Typhoon fluorescence imager. …”

Data_Sheet_1_Bullet-shaped magnetosomes and metagenomic-based magnetosome gene profiles in a deep-sea hydrothermal vent chimney.DOCX de réir Shinsaku Nakano (16454274)

“…Additionally, the electron microscopy of particles collected by magnetic separation from the chimney sample revealed MTB cells with bullet-shaped magnetosomes, and there were minor occurrences of cuboctahedral and hexagonal prismatic magnetosomes. Genome-resolved metagenomic analysis was performed to identify microorganisms that formed magnetosomes. …”

Table_1_Elucidating the Exceptional Passivation Effect of 0.8 nm Evaporated Aluminium on Transparent Copper Films.DOCX de réir Philip Bellchambers (6038882)

“…Using a combination of annular-dark field scanning transmission electron microscopy, nanoscale spatially resolved elemental analysis and atomic force microscopy, we show that this surprising effectiveness of this layer results from spontaneous segregation of the aluminium to grain boundaries in the copper film where it forms a ternary oxide plug at those sites in the metal film most vulnerable to oxidation. …”

Theta Oscillations Underpin Syntactic Encoding in First and Second Language Sentence Production de réir Zhaoqian Yue (22012613)

“…We found qualitatively distinct neural dynamics between groups. Time-resolved multivariate decoding revealed that L1 speakers formed robust and sustained complex syntactic representations from the onset of sentence planning, whereas L2 representations were delayed and transient. …”

Binding of β-catenin to the TBE Site II (−689/−684) in the COX2 promoter. de réir Felipe Nuñez (225419)

“…DNA-protein complexes formed by incubating nuclear extracts (N.E.) from MKN45 cells with radiolabelled probes containing the intact and a mutated TBE Site II (lanes 3 and 5) were resolved in native polyacrylamide gels at 5% and revealed through autoradiography. …”

Theta Oscillations Underpin Syntactic Encoding in First and Second Language Sentence Production de réir Zhaoqian Yue (22012613)

“…We found qualitatively distinct neural dynamics between groups. Time-resolved multivariate decoding revealed that L1 speakers formed robust and sustained complex syntactic representations from the onset of sentence planning, whereas L2 representations were delayed and transient. …”

The cellular automaton reproduces the cluster size distribution <i>ρ</i> of the experiments of Méhes et al. [20] for EPC with PFK closer than the Cahn-Hilliard model. de réir Florian Franke (8822846)

“…Note that clusters below 2 cells are neglected as they can not be resolved in the video, see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1010460#pcbi.1010460.s001" target="_blank">S1 Text</a>.…”

Formation of Double-Strand Dimetallic Helicates with a Terpyridine-Based Macrocycle de réir Carla Bazzicalupi (1601914)

“…The macrocyclic ligand (L), containing two terpyridine (terpy) and two ethylenediamine (en) groups arranged in a cyclic terpy-en-terpy-en sequence, forms a double-strand helicate Cu₂L⁴⁺ complex made especially stable by the formation of interstrand π–π stacking interactions involving opposite pyridine rings. …”

A Middle Palaeolithic wooden digging stick from Aranbaltza III, Spain de réir Joseba Rios-Garaizar (5029853)

“…<div><p>Aranbaltza is an archaeological complex formed by at least three open-air sites. Between 2014 and 2015 a test excavation carried out in Aranbaltza III revealed the presence of a sand and clay sedimentary sequence formed in floodplain environments, within which six sedimentary units have been identified. …”

The formyl peptide receptor agonist FPRa14 induces differentiation of Neuro2a mouse neuroblastoma cells into multiple distinct morphologies which can be specifically inhibited with... de réir Peter J. G. Cussell (6808058)

“…These three forms were also observed in the human neuroblastoma cell-lines IMR-32 and SH-SY5Y when exposed to 100μM FPRa14. …”