Combined Noncoding RNA-mRNA Regulomics Signature in Reprogramming and Pluripotency in iPSCs by Salam Salloum-Asfar (656363)

“…In this study, we performed quantitative miRNAome and small RNA-seq sequencing and applied our previously identified transcriptome to identify the potential miRNA–mRNA regulomics and regulatory network of other ncRNAs. …”

Analysis of mRNA in hemophilia A patients with undetectable mutations reveals normal splicing in the factor VIII gene by El-Maarri, O.

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T-cell-derived interleukin-17 regulates the level and stability of cyclooxygenase-2 (COX-2) mRNA through restricted activation of the p38 mitogen-activated protein kinase cascade by Faour, Wissam

“…Adding rhIL-17 to transcriptionally arrested cells stabilized COX-2 mRNA for up to 6 h, a process compromised by SB202190. …”
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Prostaglandin E2 regulates the level and stability of cyclooxygenase-2 mRNA through activation of p38 mitogen-activated protein kinase in interleukin-1β-treated human synovial fibr... by Faour, Wissam

“…This response was mediated entirely by a PGE2/p38 MAPK-dependent process. We conclude that the magnitude and duration of the induction of COX-2 mRNA, protein, and PGE2 release by rhIL-1β is primarily the result of PGE2-dependent stabilization of COX-2 mRNA and stimulation of translation, a process involving a positive feedback loop mediated by the EP4 receptor and the downstream kinases p38 MAPK and, perhaps, cAMP-dependent protein kinase.…”
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New insight into the molecular basis of hemophilia A by El Maarri, Osman

“…This fact points to possibilities of unknown gene rearrangements that disrupt the F8 gene or mutations in other genes that play a role in the processing/secretion of the factor VIII protein. Moreover, the proof of an absence of F8 messenger RNA (mRNA) in one patient points to either a defect in the expression of F8 mRNA or its rapid degradation, which may represent a novel mechanism leading to HA.…”
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Protocadherin of the liver, kidney and colon associates with detergent-resistant membranes during cellular differentiation by Rizk, Sandra

“…Several aspects of the structure, posttranslational processing, targeting, and function of this new protocadherin are still not known. …”
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Nimesulide, a preferential cyclooxygenase 2 inhibitor, suppresses peroxisome proliferator‐activated receptor induction of cyclooxygenase 2 gene expression in human synovial fibrobl... by Faour, Wissam

“…PPARα and, especially, PPARγ mediated the induction of COX-2 gene expression by receptor agonists. Stimulation of COX-2 mRNA expression and protein synthesis by 15d-PGJ2 appeared to occur through a receptor-independent process. …”
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Early growth response factor-1 mediates prostaglandin E2-dependent transcriptional suppression of cytokine-induced tumor necrosis factor-α gene expression in human macrophages and... by Faour, Wissam

“…Tumor necrosis factor-α (TNF-α) is a pleiotropic proinflammatory cytokine that modulates a broad range of inflammatory and immunological processes. We have investigated the potential immunomodulatory properties of prostaglandin E2 (PGE2) by examining the molecular mechanism by which the eicosanoid suppresses T-cell-derived interleukin-17 (IL-17)-induced TNF-α mRNA expression and protein synthesis in human macrophages and rheumatoid arthritis-affected synovial fibroblasts. …”
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Molecular Mechanisms of Adiponectin-Induced Attenuation of Mechanical Stretch-Mediated Vascular Remodeling by Crystal M. Ghantous (14571292)

“…The mRNA expression of the APN receptors AdipoR1, AdipoR2, and T-cadherin significantly increased after 15 hours of stretch. …”

The protective effects of conditioned media from human mesenchymal stem cells against Methylglyoxal-mediated injury of cultured endothelial cells by Maram Hasan (6672440)

“…We noted that only the mRNA expression of Nrf2 was increased in cells treated with MSCs-CM (50%). …”

Possible Physical Mechanisms of tRNA Pre-Selection in the Cytoplasm of Escherichia coli Bacteria by Piros, Sandor

“…The first hypothesis is that the ribosome stores tRNA molecules inside its structure and aids in the pre-selection process. Because no previous reports in literature support such a pre-selection process, we believe that this hypothesis is unlikely. …”
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Microinjection quality control in zebrafish model for genetic manipulations by Doua Abdelrahman (17280667)

“…In this technical note, in vitro transcribed synthetic mRNA encoding green fluorescence protein (eGFP), and red fluorescent protein (m-cherry) as well as fluorescein and rhodamine fluorescent dyes were injected into a single-cell zebrafish embryo for volume quality control. …”

The Effect of Sodium Butyrate and Sodium Propionate on Breast Cancer Cell Migration and Epithelial-to-Mesenchymal Transition (EMT) by Kharazi, Dania

“…Finally, treatment with NaB or NaP altered the mRNA and protein profile of EMT-associated markers, suggesting a potential reversal of the EMT process and a possible mechanism mediating the antitumor effects of the drugs. …”
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Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord by Essam M. Abdelalim (5768072)

“…RT-PCR analysis showed that BNP mRNA was present in the spinal cord and dorsal root ganglion (DRG). …”

PCF11, a Novel CD44-Downstream Transcriptional Target, Linking Its 3’-End Polyadenylation Function to Tumor Cell Metastasis by Maryam Al-Mansoob (16023220)

“…Metastasis, a multistep process, is the worst aspect of cancer and tumor cell invasion is the defining step. …”

The Impact of Acute and Chronic Exercise on Immunoglobulins and Cytokines in Elderly: Insights From a Critical Review of the Literature by Maha Sellami (4351159)

“…Strength training for 6-12 weeks did not alter plasma IL-1β, IL-2, IL-6 and TNF-α concentration in healthy elderly adults and patients with chronic-degenerative diseases, while 12 weeks of resistance training decreased muscle TNF-α mRNA in frail elderly individuals. Short-term (i.e., 10-24 weeks) moderate- to high-intensity strength training reduced LPS–IL-6, LPS, IL-1β, LPS–TNF-α and circulating concentrations of TNF-α and increased IL-10 in healthy elderly women and older people with cognitive impairment, respectively. …”

Insights Into the Role of CircRNAs: Biogenesis, Characterization, Functional, and Clinical Impact in Human Malignancies by Sabah Nisar (12561961)

“…Compared to the linear RNA, circRNAs are produced differentially by backsplicing exons or lariat introns from a pre-messenger RNA (mRNA) forming a covalently closed loop structure missing 3′ poly-(A) tail or 5′ cap, rendering them immune to exonuclease-mediated degradation. …”

Gene Expression Landscape of Chronic Myeloid Leukemia K562 Cells Overexpressing the Tumor Suppressor Gene PTPRG by Giulia Lombardi (3944720)

“…The prediction was confirmed at the mRNA and protein level, further validating the approach and identifying a new molecular mechanism of tumor suppression governed by PTPRG in a CML context.…”

Resveratrol-Elicited PKC Inhibition Counteracts NOX-Mediated Endothelial to Mesenchymal Transition in Human Retinal Endothelial Cells Exposed to High Glucose by Roberta Giordo (299595)

“…Here, we investigate whether EndMT is implicated in the diabetic retinopathy fibrotic process and evaluate the possibility that resveratrol could counteract EndMT by inhibiting high glucose (HG)-induced increases in ROS. …”