HSF2 expression sustains KSHV infection in KLEC. by Lorenza Cutrone (21144285)

Primers used in ChIP-PCR. by Lorenza Cutrone (21144285)

“…In lymphatic endothelial cells infected with KSHV and in gastric cancer cells positive for EBV, ectopic HSF2 enhances the expression of lytic genes; While knocking down HSF2 significantly decreases their expression. HSF2 overexpression is accompanied by decreased levels of repressive histone marks at the promoters of the lytic regulators KSHV ORF50 and EBV BZLF1, both characterized by poised chromatin features. …”

Primers used in RTqPCR. by Lorenza Cutrone (21144285)

“…In lymphatic endothelial cells infected with KSHV and in gastric cancer cells positive for EBV, ectopic HSF2 enhances the expression of lytic genes; While knocking down HSF2 significantly decreases their expression. HSF2 overexpression is accompanied by decreased levels of repressive histone marks at the promoters of the lytic regulators KSHV ORF50 and EBV BZLF1, both characterized by poised chromatin features. …”

HSF2 regulates ORF50 expression in an RTA independent manner. by Lorenza Cutrone (21144285)

HSF2 regulates lytic gene expression in latency and during the lytic phase. by Lorenza Cutrone (21144285)

HEK.219 cells were treated with the indicated siRNAs for 24h. by Lorenza Cutrone (21144285)

HSF2 binds the ORF50 promoter and remodels the local chromatin landscape. by Lorenza Cutrone (21144285)

Experimental design. by Amy J. Schuh (8520354)

qPCR and Western blotting of CK8 knockdown blocking the rTsSPc-activated pathway and -reduced TJs expression. by Wen Wen Zheng (17721404)

The ROCK1 pathway inhibitor Y27632 prevented rTsSPc from activating the ROCK1 pathway and reducing TJs expression. by Wen Wen Zheng (17721404)

PAS staining of intestinal sections from <i>T. spiralis</i>-infected mice pretreated with DAS and Y27632. by Wen Wen Zheng (17721404)

Effects of SGLT-2is during the time course. by Ningning Wang (560944)

HSF2 is degraded during KSHV lytic reactivation. by Lorenza Cutrone (21144285)

IFA, GST pull-down and Co-IP assay of specific binding between rTsSPc and CK8. by Wen Wen Zheng (17721404)

HE staining showed that DAS and Y27632 alleviated intestinal inflammation in <i>T. spiralis</i>-infected mice. by Wen Wen Zheng (17721404)

HSF2 binds to the BZLF1 promoter and remodels the chromatin locally. by Lorenza Cutrone (21144285)

Transcription and expression levels of CK8 in Caco-2 cells after CK8 gene was knocked down. by Wen Wen Zheng (17721404)

Spontaneous physical activity and respiratory quotient (RQ) in WT and NMIIC KO mice. by Hiroki Hamaguchi (22783780)

sRAGE accelerates regeneration in aged skeletal muscle after cryoinjury. by Naftali Horwitz (3130086)

“…Dots represent data for individual animals overlaid with mean ±  SD. Data analyzed for statistical significance by one-way ANOVA with Tukey post hoc test (A, D, G) or Student’s two-tailed unpaired t test (E, H, J, K, L, M). …”

(a) 48 sandstone samples used in this experiment, (b) ZYB-II vacuum pressurized water saturation device, (c)static strain gauge, (d) diagram of experimental system, including uniax... by Wenyu Lv (20139458)