Optimisation of read depth, DNA quantity, and unique alternate observation threshold. by Melinda L. Tursky (20790436)

“…The mean number of variants detected per sample above the standard CHIP threshold of ≥ 0.02 VAF increased linearly with subject age (E). Greater than 99.9% of variants detected were below ≥ 0.02 VAF, potentially including those with prognostic or predictive clinical relevance (F). …”