Integrated Transcriptome and Pathway Analyses Revealed Multiple Activated Pathways in Breast Cancer by Radhakrishnan Vishnubalaji (3563306)

“…A selected number of genes from the RNA-Seq analysis were subsequently validated using qRT-PCR. …”

Single-Cell Transcriptome Analysis Highlights a Role for Neutrophils and Inflammatory Macrophages in the Pathogenesis of Severe COVID-19 by Hibah Shaath (5599658)

“…Interestingly, although macrophages were also present in healthy subjects and patients with mild COVID-19, they had different gene signatures, indicative of interstitial and cluster-0 macrophage (i.e., FABP4, APOC1, APOE, C1QB, and NURP1). …”

Integrated transcriptional‐phenotypic analysis captures systemic immunomodulation following antiangiogenic therapy in renal cell carcinoma patients by Darawan Rinchai (742366)

Single-cell transcriptomics identifies master regulators of neurodegeneration in SOD1 ALS iPSC-derived motor neurons by Seema C. Namboori (11031624)

“…Specifically, we identified activation of SMAD2, a downstream mediator of the transforming growth factor β (TGF-β) signaling pathway as a key driver of SOD1 iPSC-derived motor neuron degeneration. Importantly, our analysis indicates that activation of TGFβ signaling may be a common mechanism shared between SOD1, FUS, C9ORF72, VCP, and sporadic ALS motor neurons. …”

Transcriptome profiling and network enrichment analyses identify subtype-specific therapeutic gene targets for breast cancer and their microRNA regulatory networks by Ramesh Elango (7542068)

“…<p dir="ltr">Previous studies have suggested that breast cancer (BC) from the Middle East and North Africa (MENA) is presented at younger age with advanced tumor stage, indicating underlying biological differences. Given the scant transcriptomic data on BC from the MENA region and to better understand the biology of this disease, we performed mRNA and microRNA (miRNA) transcriptomic profiling on a local cohort of BC (<i>n</i> = 96) from Qatar. …”

The Potential Role of Thyroid Hormone Therapy in Neural Progenitor Cell Differentiation and Its Impact on Neurodevelopmental Disorders by Salam Salloum-Asfar (656363)

“…W6 and W8 neurons exhibited higher action potential frequencies, with W6 showing increased peak amplitudes and shortened inter-spike intervals by 50%, indicating enhanced activity. Transcriptomic analysis revealed that W6 T3-treated neurons formed a distinct cluster, suggesting accelerated maturation. …”

Long non-coding RNA MALAT1 promotes steatosis in HepG2 cells by modulating target gene expression in presence of Exendin-4 by Olfa Khalifa (10914452)

“…Furthermore, confocal microscopy analysis of BODIPY-stained untreated, steatotic, and Ex-4-treated steatotic cells showed that Ex-4 significantly decreases the number of lipid droplets. …”

Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency by Ahmed K. Elsayed (13275302)

“…At pancreatic progenitor (PP2) and endocrine progenitor (EPs) stages, transcriptome analysis showed downregulation in genes associated with pancreatic development and diabetes and upregulation in genes associated with nervous system development and WNT signaling pathway. …”

iPSC-Derived Pancreatic Progenitors Lacking FOXA2 Reveal Alterations in miRNA Expression Targeting Key Pancreatic Genes by Noura Aldous (15429873)

“…RT-qPCR was performed for validating selected DEGs and miRNAs. </p> <p>Results: Transcriptome analysis on FOXA2–/–PPs compared to WT-PPs revealed 1628 down-regulated (Log2 FC < −1.0, p < 0.05) and 769 up-regulated (Log2 FC > 1.0, p < 0.05) DEGs. …”

Long non-coding RNA AC099850.4 correlates with advanced disease state and predicts worse prognosis in triple-negative breast cancer by Radhakrishnan Vishnubalaji (3563306)

“…Among those, AC099850.4 lncRNA was chosen for further investigation where it exhibited elevated expression, which was further confirmed in a second TNBC cohort (<i>n</i> = 360) where its expression correlated with a worse prognosis. Network analysis of AC099850.4^high TNBC highlighted enrichment in functional categories indicative of cell cycle activation and mitosis. …”

Enhanced generation of beta cell precursors and functional insulin-secreting cells from human pluripotent stem cells by Bushra Memon (4792767)

“…The progenitors and beta cells were extensively characterized for gene expression by immunofluorescence, RT-PCR and functional assay. Transcriptome analysis was performed at different stages and compared with profiles of various human pancreatic counterparts.…”

Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis by Muneera Vakayil (12037693)

“…</p> <p>Methods: We performed CRISPR/ Cas9 and shRNA-mediated gene silencing in addition to whole transcriptomics analysis on Uhrf1 KO cells.</p> <p>Results: We identified altered molecular and cellular pathways indicating that Uhrf1 is a critical factor required for adipocyte metabolism, and in its absence, cells exhibit adipocyte dysfunctions with higher levels of inflammatory markers.…”

PDX1⁻/NKX6.1⁺ progenitors derived from human pluripotent stem cells as a novel source of insulin‐secreting cells by Bushra Memon (4792767)

“…The progenitors and beta cells were extensively characterized. Transcriptome analysis was performed at different stages and compared with the profiles of various pancreatic counterparts.…”