DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014)
DJ-1 is a 20-KDa protein that was first identified as an oncogene product responsible for a subset of familial Parkinson’s disease; hence its name PARK 7. Previous studies showed that DJ-1 negatively regulated the tumor suppressor gene PTEN expression; thus promoting the phosphorylation of the PI3K/...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | masterThesis |
| منشور في: |
2016
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3258 https://doi.org/10.26756/th.2014.62 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513460923006976 |
|---|---|
| author | Yassine, Rawan M. |
| author_facet | Yassine, Rawan M. |
| author_role | author |
| dc.creator.none.fl_str_mv | Yassine, Rawan M. |
| dc.date.none.fl_str_mv | 2016-03-03T13:17:44Z 2016-03-03T13:17:44Z 2016-03-03 5/16/2014 |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/3258 https://doi.org/10.26756/th.2014.62 |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Rho GTPases Cells -- Motility Antioncogenes Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | DJ-1 is a 20-KDa protein that was first identified as an oncogene product responsible for a subset of familial Parkinson’s disease; hence its name PARK 7. Previous studies showed that DJ-1 negatively regulated the tumor suppressor gene PTEN expression; thus promoting the phosphorylation of the PI3K/Akt signaling pathway, and activating cell proliferation and transformation. Therefore, DJ-1 can be used as an indication of cancer metastasis and can be a potential therapeutic target. However, the localization, detailed mechanism and the role of DJ-1 in cellular motility are still not fully understood. Therefore, our study showed that DJ-1, at normal cellular condition, is present in the nucleus, mitochondria, golgi apparatus and plasma membrane of astrocytoma (SF268) cells. Upon its stimulation with EGF, DJ-1 localize outside the nucleus, leading to an increase in cellular motility in lung, brain, and breast cancer cells by activating the PI3K pathway. We also showed that this activation is carried out by inhibiting PTEN and thus regulating the RhoGTPases mainly Rac which is known to be involved in cellular motility. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_6c646e8e8ae8cd6978d78e0626e91d78 |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3258 |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014)Yassine, Rawan M.Rho GTPasesCells -- MotilityAntioncogenesLebanese American University -- DissertationsDissertations, AcademicDJ-1 is a 20-KDa protein that was first identified as an oncogene product responsible for a subset of familial Parkinson’s disease; hence its name PARK 7. Previous studies showed that DJ-1 negatively regulated the tumor suppressor gene PTEN expression; thus promoting the phosphorylation of the PI3K/Akt signaling pathway, and activating cell proliferation and transformation. Therefore, DJ-1 can be used as an indication of cancer metastasis and can be a potential therapeutic target. However, the localization, detailed mechanism and the role of DJ-1 in cellular motility are still not fully understood. Therefore, our study showed that DJ-1, at normal cellular condition, is present in the nucleus, mitochondria, golgi apparatus and plasma membrane of astrocytoma (SF268) cells. Upon its stimulation with EGF, DJ-1 localize outside the nucleus, leading to an increase in cellular motility in lung, brain, and breast cancer cells by activating the PI3K pathway. We also showed that this activation is carried out by inhibiting PTEN and thus regulating the RhoGTPases mainly Rac which is known to be involved in cellular motility.N/A1 hard copy: xi, 55 leaves; ill.; 30 cm. available at RNL.Includes bibliographical references (leaves 43-55).Lebanese American University2016-03-03T13:17:44Z2016-03-03T13:17:44Z5/16/20142016-03-03Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/3258https://doi.org/10.26756/th.2014.62eninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/32582023-11-08T09:47:18Z |
| spellingShingle | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) Yassine, Rawan M. Rho GTPases Cells -- Motility Antioncogenes Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| title_full | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| title_fullStr | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| title_full_unstemmed | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| title_short | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| title_sort | DJ-1, a peten inhibitor, is a positive regulator of cancer cell motility. (c2014) |
| topic | Rho GTPases Cells -- Motility Antioncogenes Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/3258 https://doi.org/10.26756/th.2014.62 |