Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes
β-Adrenergic stimulation increases cAMP in cardiac cells to induce PKA-mediated phosphorylation of various proteins regulating heart function, such as the L-type Ca 2+ channels which carry I Ca,L and trigger cardiac contraction. Phosphodiesterases (PDEs) 3 and 4 are the main cAMP degrading enzymes i...
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2007
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| Online Access: | http://hdl.handle.net/10725/6362 http://dx.doi.org/10.1117/j.yjmcc.2007.03.139 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jmmc-online.com/article/S0022-2828(07)00195-2/abstract#articleInformation |
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| _version_ | 1864513479750189056 |
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| author | Leroy, Jerome |
| author2 | Abi-Gerges, Aniella Richter, Wito Nikolaev, Viacheslav O. Engelhardt, Stefan Lohse, Martin J. Conti, Marco Fischmeister, Rodolphe Vandecasteele, Gregoire |
| author2_role | author author author author author author author author |
| author_facet | Leroy, Jerome Abi-Gerges, Aniella Richter, Wito Nikolaev, Viacheslav O. Engelhardt, Stefan Lohse, Martin J. Conti, Marco Fischmeister, Rodolphe Vandecasteele, Gregoire |
| author_role | author |
| dc.creator.none.fl_str_mv | Leroy, Jerome Abi-Gerges, Aniella Richter, Wito Nikolaev, Viacheslav O. Engelhardt, Stefan Lohse, Martin J. Conti, Marco Fischmeister, Rodolphe Vandecasteele, Gregoire |
| dc.date.none.fl_str_mv | 2007 2017-10-19T12:43:47Z 2017-10-19T12:43:47Z 2017-10-19 |
| dc.identifier.none.fl_str_mv | 0022-2828 http://hdl.handle.net/10725/6362 http://dx.doi.org/10.1117/j.yjmcc.2007.03.139 Leroy, J., Abi-Gerges, A., Richter, W., Nikolaev, V. O., Engelhardt, S., Lohse, M. J., ... & Vandecasteele, G. (2007). Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa, L in adult rat ventricular myocytes. Journal of Molecular and Cellular Cardiology, 42(6), S49. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jmmc-online.com/article/S0022-2828(07)00195-2/abstract#articleInformation |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Molecular and Cellular Cardiology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | β-Adrenergic stimulation increases cAMP in cardiac cells to induce PKA-mediated phosphorylation of various proteins regulating heart function, such as the L-type Ca 2+ channels which carry I Ca,L and trigger cardiac contraction. Phosphodiesterases (PDEs) 3 and 4 are the main cAMP degrading enzymes in cardiomyocytes and modulate the β-adrenergic regulation of I Ca,L but the significance of this dual regulation remains elusive. Here, we compared the participation of PDE3 and PDE4 in the β-adrenergic regulation of intracellular cAMP and I Ca,L using the FRET-based indicator Epac2-cAMPS and the whole-cell patch-clamp technique in adult rat ventricular myocytes. Isoprenaline (Iso, 100 nM, 15 s) induced a transient increase in both [cAMP] i and I Ca,L. PDE3 inhibition with cilostamide (1 μM) neither modified [cAMP] i nor I Ca,L transients, whereas PDE4 inhibition with Ro-201724 (10 μM) prolonged both signals. When both PDE3 and PDE4 were blocked, [cAMP] i and I Ca,L signals became nearly stable. PKA inhibition with PKI prolonged the β-adrenergic cAMP transient and this effect was not additive with that of Ro201724. Thus, PDE4 is predominant to terminate β-adrenergic induced [cAMP] i increase and I Ca,L stimulation in adult rat cardiomyocytes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_e93efcc919cdd2fc0f9bf14c98278f6b |
| identifier_str_mv | 0022-2828 Leroy, J., Abi-Gerges, A., Richter, W., Nikolaev, V. O., Engelhardt, S., Lohse, M. J., ... & Vandecasteele, G. (2007). Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa, L in adult rat ventricular myocytes. Journal of Molecular and Cellular Cardiology, 42(6), S49. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6362 |
| publishDate | 2007 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytesLeroy, JeromeAbi-Gerges, AniellaRichter, WitoNikolaev, Viacheslav O.Engelhardt, StefanLohse, Martin J.Conti, MarcoFischmeister, RodolpheVandecasteele, Gregoireβ-Adrenergic stimulation increases cAMP in cardiac cells to induce PKA-mediated phosphorylation of various proteins regulating heart function, such as the L-type Ca 2+ channels which carry I Ca,L and trigger cardiac contraction. Phosphodiesterases (PDEs) 3 and 4 are the main cAMP degrading enzymes in cardiomyocytes and modulate the β-adrenergic regulation of I Ca,L but the significance of this dual regulation remains elusive. Here, we compared the participation of PDE3 and PDE4 in the β-adrenergic regulation of intracellular cAMP and I Ca,L using the FRET-based indicator Epac2-cAMPS and the whole-cell patch-clamp technique in adult rat ventricular myocytes. Isoprenaline (Iso, 100 nM, 15 s) induced a transient increase in both [cAMP] i and I Ca,L. PDE3 inhibition with cilostamide (1 μM) neither modified [cAMP] i nor I Ca,L transients, whereas PDE4 inhibition with Ro-201724 (10 μM) prolonged both signals. When both PDE3 and PDE4 were blocked, [cAMP] i and I Ca,L signals became nearly stable. PKA inhibition with PKI prolonged the β-adrenergic cAMP transient and this effect was not additive with that of Ro201724. Thus, PDE4 is predominant to terminate β-adrenergic induced [cAMP] i increase and I Ca,L stimulation in adult rat cardiomyocytes.PublishedN/A2017-10-19T12:43:47Z2017-10-19T12:43:47Z20072017-10-19Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0022-2828http://hdl.handle.net/10725/6362http://dx.doi.org/10.1117/j.yjmcc.2007.03.139Leroy, J., Abi-Gerges, A., Richter, W., Nikolaev, V. O., Engelhardt, S., Lohse, M. J., ... & Vandecasteele, G. (2007). Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa, L in adult rat ventricular myocytes. Journal of Molecular and Cellular Cardiology, 42(6), S49.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.jmmc-online.com/article/S0022-2828(07)00195-2/abstract#articleInformationenJournal of Molecular and Cellular Cardiologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/63622021-03-19T10:43:10Z |
| spellingShingle | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes Leroy, Jerome |
| status_str | publishedVersion |
| title | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| title_full | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| title_fullStr | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| title_full_unstemmed | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| title_short | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| title_sort | Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes |
| url | http://hdl.handle.net/10725/6362 http://dx.doi.org/10.1117/j.yjmcc.2007.03.139 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jmmc-online.com/article/S0022-2828(07)00195-2/abstract#articleInformation |