Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System
<div><p>The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene. CRISPR components are typically delivered to cells as either a Cas9/sgRNA ribonucleoprotein (RNP) complex or a pl...
محفوظ في:
| المؤلف الرئيسي: | Karim E. Shalaby (12267224) (author) |
|---|---|
| مؤلفون آخرون: | Mustapha Aouida (417652) (author), Vijay Gupta (209146) (author), Simona S. Ghanem (12267227) (author), Omar M. A. El-Agnaf (8809331) (author) |
| منشور في: |
2022
|
| الموضوعات: | |
| الوسوم: |
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