Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy
<div><p>Interactions between programmed death-1 (PD-1) with its ligand PD-L1 on tumor cells can antagonize T cell responses. Inhibiting these interactions using immune checkpoint inhibitors has shown promise in cancer immunotherapy. MDA-MB-231 is a triple negative breast cancer cell line...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2019
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| الموضوعات: | |
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| _version_ | 1864513521126998016 |
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| author | Mohamed H. M. Ali (5768066) |
| author2 | Salman M Toor (9279953) Fazle Rakib (5768069) Raghvendra Mall (581171) Ehsan Ullah (2698921) Kamal Mroue (14152491) Prasanna R. Kolatkar (124118) Khalid Al-Saad (5768084) Eyad Elkord (5396390) |
| author2_role | author author author author author author author author |
| author_facet | Mohamed H. M. Ali (5768066) Salman M Toor (9279953) Fazle Rakib (5768069) Raghvendra Mall (581171) Ehsan Ullah (2698921) Kamal Mroue (14152491) Prasanna R. Kolatkar (124118) Khalid Al-Saad (5768084) Eyad Elkord (5396390) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed H. M. Ali (5768066) Salman M Toor (9279953) Fazle Rakib (5768069) Raghvendra Mall (581171) Ehsan Ullah (2698921) Kamal Mroue (14152491) Prasanna R. Kolatkar (124118) Khalid Al-Saad (5768084) Eyad Elkord (5396390) |
| dc.date.none.fl_str_mv | 2019-09-09T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/vaccines7030109 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Investigation_of_the_Effect_of_PD-L1_Blockade_on_Triple_Negative_Breast_Cancer_Cells_Using_Fourier_Transform_Infrared_Spectroscopy/25907587 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumor cells spectroscopy FTIR biochemical alterations chemometric analysis |
| dc.title.none.fl_str_mv | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Interactions between programmed death-1 (PD-1) with its ligand PD-L1 on tumor cells can antagonize T cell responses. Inhibiting these interactions using immune checkpoint inhibitors has shown promise in cancer immunotherapy. MDA-MB-231 is a triple negative breast cancer cell line that expresses PD-L1. In this study, we investigated the biochemical changes in MDA-MB-231 cells following treatment with atezolizumab, a specific PD-L1 blocker. Our readouts were Fourier Transform Infrared (FTIR) spectroscopy and flow cytometric analyses. Chemometrical analysis, such as principal component analysis (PCA), was applied to delineate the spectral differences. We were able to identify the chemical alterations in both protein and lipid structure of the treated cells. We found that there was a shift from random coil and α-helical structure to β-sheet conformation of PD-L1 on tumor cells due to atezolizumab treatment, which could hinder binding with its receptors on immune cells, ensuring sustained T cell activation for potent immune responses. This work provides novel information about the effects of atezolizumab at molecular and cellular levels. FTIR bio-spectroscopy, in combination with chemometric analyses, may expedite research and offer new approaches for cancer immunology.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Vaccines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/vaccines7030109" target="_blank">https://dx.doi.org/10.3390/vaccines7030109</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_66b2aa409d33a9910afff8ac2766580e |
| identifier_str_mv | 10.3390/vaccines7030109 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25907587 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared SpectroscopyMohamed H. M. Ali (5768066)Salman M Toor (9279953)Fazle Rakib (5768069)Raghvendra Mall (581171)Ehsan Ullah (2698921)Kamal Mroue (14152491)Prasanna R. Kolatkar (124118)Khalid Al-Saad (5768084)Eyad Elkord (5396390)Biomedical and clinical sciencesOncology and carcinogenesisbreast cancertumor cellsspectroscopyFTIRbiochemical alterationschemometric analysis<div><p>Interactions between programmed death-1 (PD-1) with its ligand PD-L1 on tumor cells can antagonize T cell responses. Inhibiting these interactions using immune checkpoint inhibitors has shown promise in cancer immunotherapy. MDA-MB-231 is a triple negative breast cancer cell line that expresses PD-L1. In this study, we investigated the biochemical changes in MDA-MB-231 cells following treatment with atezolizumab, a specific PD-L1 blocker. Our readouts were Fourier Transform Infrared (FTIR) spectroscopy and flow cytometric analyses. Chemometrical analysis, such as principal component analysis (PCA), was applied to delineate the spectral differences. We were able to identify the chemical alterations in both protein and lipid structure of the treated cells. We found that there was a shift from random coil and α-helical structure to β-sheet conformation of PD-L1 on tumor cells due to atezolizumab treatment, which could hinder binding with its receptors on immune cells, ensuring sustained T cell activation for potent immune responses. This work provides novel information about the effects of atezolizumab at molecular and cellular levels. FTIR bio-spectroscopy, in combination with chemometric analyses, may expedite research and offer new approaches for cancer immunology.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Vaccines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/vaccines7030109" target="_blank">https://dx.doi.org/10.3390/vaccines7030109</a></p>2019-09-09T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/vaccines7030109https://figshare.com/articles/journal_contribution/Investigation_of_the_Effect_of_PD-L1_Blockade_on_Triple_Negative_Breast_Cancer_Cells_Using_Fourier_Transform_Infrared_Spectroscopy/25907587CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259075872019-09-09T03:00:00Z |
| spellingShingle | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy Mohamed H. M. Ali (5768066) Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumor cells spectroscopy FTIR biochemical alterations chemometric analysis |
| status_str | publishedVersion |
| title | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| title_full | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| title_fullStr | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| title_full_unstemmed | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| title_short | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| title_sort | Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy |
| topic | Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumor cells spectroscopy FTIR biochemical alterations chemometric analysis |