A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus

A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus

<p>PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion. The first stage of the restriction enzyme assay is hybridization of a target DNA...

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Bibliographic Details
Main Author: Maria W. Smith (18807382) (author)
Other Authors: Andrei L. Ghindilis (18807385) (author), Ihab A. Seoudi (18807388) (author), Kenneth Smith (149075) (author), Rosalind Billharz (565086) (author), Holly M. Simon (239888) (author)
Published: 2014
Subjects:
Biological sciences
Genetics
Biomedical and clinical sciences
Medical biotechnology
Polymerase chain reaction
Oligonucleotides
Enzyme assays
Point mutation
Methicillin-resistant Staphylococcus aureus
Probe hybridization
Bacterial pathogens
Genotyping
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