Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study
<div><p>Genome-wide association studies (GWAS) with non-targeted metabolomics have identified many genetic loci of biomedical interest. However, metabolites with a high degree of missingness, such as drug metabolites and xenobiotics, are often excluded from such studies due to a lack of...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2022
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| _version_ | 1864513519114780672 |
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| author | Gaurav Thareja (459188) |
| author2 | Anne M. Evans (127464) Spencer D. Wood (18418539) Nisha Stephan (17445912) Shaza Zaghlool (3466298) Anna Halama (545988) Gabi Kastenmüller (127487) Aziz Belkadi (779313) Omar M. E. Albagha (11704871) Karsten Suhre (67967) The Qatar Genome Program Research Consortium (14778616) |
| author2_role | author author author author author author author author author author |
| author_facet | Gaurav Thareja (459188) Anne M. Evans (127464) Spencer D. Wood (18418539) Nisha Stephan (17445912) Shaza Zaghlool (3466298) Anna Halama (545988) Gabi Kastenmüller (127487) Aziz Belkadi (779313) Omar M. E. Albagha (11704871) Karsten Suhre (67967) The Qatar Genome Program Research Consortium (14778616) |
| author_role | author |
| dc.creator.none.fl_str_mv | Gaurav Thareja (459188) Anne M. Evans (127464) Spencer D. Wood (18418539) Nisha Stephan (17445912) Shaza Zaghlool (3466298) Anna Halama (545988) Gabi Kastenmüller (127487) Aziz Belkadi (779313) Omar M. E. Albagha (11704871) Karsten Suhre (67967) The Qatar Genome Program Research Consortium (14778616) |
| dc.date.none.fl_str_mv | 2022-05-30T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/metabo12060496 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Ratios_of_Acetaminophen_Metabolites_Identify_New_Loci_of_Pharmacogenetic_Relevance_in_a_Genome-Wide_Association_Study/25658886 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences medication drug metabolites population studies non-targeted metabolomics genome-wide association studies acetaminophen metabolism pharmacogenomics 3-methoxyacetaminophen |
| dc.title.none.fl_str_mv | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Genome-wide association studies (GWAS) with non-targeted metabolomics have identified many genetic loci of biomedical interest. However, metabolites with a high degree of missingness, such as drug metabolites and xenobiotics, are often excluded from such studies due to a lack of statistical power and higher uncertainty in their quantification. Here we propose ratios between related drug metabolites as GWAS phenotypes that can drastically increase power to detect genetic associations between pairs of biochemically related molecules. As a proof-of-concept we conducted a GWAS with 520 individuals from the Qatar Biobank for who at least five of the nine available acetaminophen metabolites have been detected. We identified compelling evidence for genetic variance in acetaminophen glucuronidation and methylation by UGT2A15 and COMT, respectively. Based on the metabolite ratio association profiles of these two loci we hypothesized the chemical structure of one of their products or substrates as being 3-methoxyacetaminophen, which we then confirmed experimentally. Taken together, our study suggests a novel approach to analyze metabolites with a high degree of missingness in a GWAS setting with ratios, and it also demonstrates how pharmacological pathways can be mapped out using non-targeted metabolomics measurements in large population-based studies.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Metabolites<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo12060496" target="_blank">https://dx.doi.org/10.3390/metabo12060496</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_ad4abfcf960977a2cf2fc10db58d65c4 |
| identifier_str_mv | 10.3390/metabo12060496 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25658886 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association StudyGaurav Thareja (459188)Anne M. Evans (127464)Spencer D. Wood (18418539)Nisha Stephan (17445912)Shaza Zaghlool (3466298)Anna Halama (545988)Gabi Kastenmüller (127487)Aziz Belkadi (779313)Omar M. E. Albagha (11704871)Karsten Suhre (67967)The Qatar Genome Program Research Consortium (14778616)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesClinical sciencesmedicationdrug metabolitespopulation studiesnon-targeted metabolomicsgenome-wide association studiesacetaminophen metabolismpharmacogenomics3-methoxyacetaminophen<div><p>Genome-wide association studies (GWAS) with non-targeted metabolomics have identified many genetic loci of biomedical interest. However, metabolites with a high degree of missingness, such as drug metabolites and xenobiotics, are often excluded from such studies due to a lack of statistical power and higher uncertainty in their quantification. Here we propose ratios between related drug metabolites as GWAS phenotypes that can drastically increase power to detect genetic associations between pairs of biochemically related molecules. As a proof-of-concept we conducted a GWAS with 520 individuals from the Qatar Biobank for who at least five of the nine available acetaminophen metabolites have been detected. We identified compelling evidence for genetic variance in acetaminophen glucuronidation and methylation by UGT2A15 and COMT, respectively. Based on the metabolite ratio association profiles of these two loci we hypothesized the chemical structure of one of their products or substrates as being 3-methoxyacetaminophen, which we then confirmed experimentally. Taken together, our study suggests a novel approach to analyze metabolites with a high degree of missingness in a GWAS setting with ratios, and it also demonstrates how pharmacological pathways can be mapped out using non-targeted metabolomics measurements in large population-based studies.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Metabolites<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo12060496" target="_blank">https://dx.doi.org/10.3390/metabo12060496</a></p>2022-05-30T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/metabo12060496https://figshare.com/articles/journal_contribution/Ratios_of_Acetaminophen_Metabolites_Identify_New_Loci_of_Pharmacogenetic_Relevance_in_a_Genome-Wide_Association_Study/25658886CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256588862022-05-30T03:00:00Z |
| spellingShingle | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study Gaurav Thareja (459188) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences medication drug metabolites population studies non-targeted metabolomics genome-wide association studies acetaminophen metabolism pharmacogenomics 3-methoxyacetaminophen |
| status_str | publishedVersion |
| title | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| title_full | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| title_fullStr | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| title_full_unstemmed | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| title_short | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| title_sort | Ratios of Acetaminophen Metabolites Identify New Loci of Pharmacogenetic Relevance in a Genome-Wide Association Study |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences medication drug metabolites population studies non-targeted metabolomics genome-wide association studies acetaminophen metabolism pharmacogenomics 3-methoxyacetaminophen |