Purification of proteolytically active recombinant HtrA proteins and assessment in protease activity assays.

Purification of proteolytically active recombinant HtrA proteins and assessment in protease activity assays.

<p>(A) Recombinant HtrA-H6 or catalytically inactive HtrA<sup>S237A</sup>-H6 were purified from <i>E. coli</i> BL21(DE3) gold via Ni-NTA resin. Eluted fractions were assessed via Western blot using rabbit serum raised against recombinant HtrA<sup>S237A</sup>...

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গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক:	Alyssa Brokaw (16469975) (author)
অন্যান্য লেখক:	Grace Wallen (16469984) (author), Austyn Orvis (16469972) (author), Hei Joon Kwon (22379320) (author), Ravin Seepersaud (243884) (author), Shayla Nguyen (16469978) (author), Kavita Sharma (1951042) (author), Michelle Coleman (621076) (author), Phoenicia Quach (8334063) (author), Joy Twentyman (5871227) (author), Jay Vornhagen (472132) (author), Lisa A. Jones (1903474) (author), Chenwei Lin (189567) (author), Philip R. Gafken (821162) (author), Lakshmi Rajagopal (247524) (author)
প্রকাশিত:	2025
বিষয়গুলি:	Medicine Microbiology Molecular Biology Pharmacology Immunology Infectious Diseases Virology Computational Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified understand transcriptional regulation family serine proteases group b streptococcus >) increased dissemination div >< p adverse pregnancy outcomes gbs infection rates streptococcus </ increased concentration wt ). women worldwide vitro </ varied mechanisms translational regulator stress responses significant proportion secreted proteins rectovaginally colonizes preterm births pregnant adults perinatal pathogen part explain neutrophil myeloperoxidase neonatal infections much progress many aspects leading cause isogenic wt isogenic wild invasive infections inflammatory il immunosuppressive il host response gbs membrane findings support endogenous surface contributing annually component systems although deletion also increasing >, highlighting
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