The nuclease activities of MRN complex participate in regulating the formation of HBV cccDNA.
<p>HepG2-NTCP-K7 cells were treated with Mirin (A-G) or PFM01 (H-N) as shown by the experimental layout (A and H). Samples were harvested at 5 days post infection (dpi) to detect. (B and I) The effects of Mirin or PFM01 on cell viabilities were detected by CCK-8 assay. (C and J) HBeAg in the c...
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2025
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| Summary: | <p>HepG2-NTCP-K7 cells were treated with Mirin (A-G) or PFM01 (H-N) as shown by the experimental layout (A and H). Samples were harvested at 5 days post infection (dpi) to detect. (B and I) The effects of Mirin or PFM01 on cell viabilities were detected by CCK-8 assay. (C and J) HBeAg in the culture medium was detected by ELISA. HBV pgRNA (D and K) and total RNA (E and L) were detected by RT-PCR assay. HBV cccDNA was detected by qPCR assay (F and M) and Southern blotting (G and N), the numbers below the blot indicate the arbitrary units from the densitometry analysis of cccDNA bands. *: p <0.05, **: p <0.01, ***: p <0.001.</p> |
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