VDAC1-MCU channels mediate mitochondrial Ca2+ entry and mitochondrial fission.

(A-H) Marc-145 cells were infected with PRRSV (MOI = 0.1) for 24 h with or without VBIT-12 (A-D) or MCU-i4 (E-H) treatment. (A and E) Ca2+ was detected by flow cytometry after Rhod-2 staining. (B and F) The cells were analyzed by confocal microscopy after staining wit...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Zhe Sun (328682) (author)
مؤلفون آخرون:	Zicheng Ma (14071216) (author), Wandi Cao (20553288) (author), Chenlong Jiang (14071219) (author), Lei Guo (36221) (author), Kesen Liu (9086343) (author), Yanni Gao (6250973) (author), Juan Bai (798838) (author), Jiang Pi (817619) (author), Ping Jiang (243020) (author), Xing Liu (221084) (author)
منشور في:	2025
الموضوعات:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Science Policy Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified “ powerhouse ” providing new insights promoting cellular glycolysis mams ), mediated ip3r )— voltage generating cellular energy excessive lactate production camkkβ )— amp ampk )— dynamin activated protein kinase regulates mitochondrial function mitochondrial dynamics proteins hijack mitochondrial function promote mitochondrial fission mediated mitochondrial fission induced mitochondrial fission induce mitochondrial fission alongside mitochondrial fission related protein 1 triggers mitophagy via mitophagy drive glycolysis respiratory syndrome virus div >< p 2 +</ sup elevated mitochondria ca mitochondrial ca inositol 1 elevated levels vital role upregulated expression triphosphate receptor study reveals sigmar1 ). sigma non mitochondria ca increased mitochondria evolved mechanisms endoplasmic reticulum dynamic processes 49 kda
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الملخص:	<p>(A-H) Marc-145 cells were infected with PRRSV (MOI = 0.1) for 24 h with or without VBIT-12 (A-D) or MCU-i4 (E-H) treatment. (A and E) Ca<sup>2+</sup> was detected by flow cytometry after Rhod-2 staining. (B and F) The cells were analyzed by confocal microscopy after staining with MitoTracker Red, PRRSV-N antibody and DAPI. The mitochondrial length was quantified from 25 cells per group (right panel). (C and G) The cell lysates were harvested for western blot analysis with antibodies against p-DRP1(S637), p-DRP1(S616), DRP1, PRRSV-N and β-actin. The levels of phosphorylated DRP1 were normalized to the total DRP1 protein, while the levels of other proteins were normalized to β-actin. (D and H) Quantification of the TCID<sub>50</sub> of PRRSV in cell supernatants. Data are expressed as means ± SD, n = 3 in A, D, E and H or n = 25 in B and F. p<0.05; p < 0.01; **p < 0.001. The data are representative of results from three independent experiments.</p>

VDAC1-MCU channels mediate mitochondrial Ca<sup>2+</sup> entry and mitochondrial fission.

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