Infectivity and binding of HAstV in wild-type, FcRn-KO, and DPP4-KO Caco-2 cells. A) Western blot showing the loss of expression of the FcRn receptor in FcRn-knockout (FcRn-KO), DPP4 in DPP4-KO cells, and of both cellular molecules in the double-KO Caco-2 cells. The arrow points to a nonspecific band revealed with the FcRn antibody. Tubulin was used as a loading control in the gel. B) Infectivity of HAst-V serotypes 1, 2, and 8, and of the divergent HAstV-VA1 in WT and three knockout Caco-2 cell lines. The infectious virus titers of HAstV-infected cell lysates were determined in Caco-2 cell monolayers grown in 96-well plates. C) Purified HAstV-1 binding in WT and the three knockout Caco-2 cell lines. Purified virus (160 ng) was added for 1 h at 4°C to allow the virus to attach to the cell surface. After removing the unbound virus and washing the cells, the attached virus was detected by RT-qPCR, as described in the Materials and Methods section. Experiments were performed on ice to

<p>Infectivity and binding of HAstV in wild-type, FcRn-KO, and DPP4-KO Caco-2 cells. A) Western blot showing the loss of expression of the FcRn receptor in FcRn-knockout (FcRn-KO), DPP4 in DPP4-KO cells, and of both cellular molecules in the double-KO Caco-2 cells. The arrow points to a nonspe...

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