Sequential elution of mono- and di-N-glycosylated rmDNase1 from hydroxyapatite.

<p>(A) Elution profile of HAP chromatography with rmDNase1 after P-100 gel-filtration as evaluated by silver gel analysis. In contrast to the experiment presented in S7 Fig, an elution step with 25 mM sodium phosphate, pH 6.8 (E25) was included. The eluate E25 contained only di-N-glycosylated...

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Bibliographic Details
Main Author:	Jan-Ole Krischek (21213122) (author)
Other Authors:	Hans Georg Mannherz (10226546) (author), Markus Napirei (10226549) (author)
Published:	2025
Subjects:	Biochemistry Genetics Molecular Biology Biotechnology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified systemic lupus erythematosus rough endoplasmic reticulum related waste disposal major endonuclease involved important therapeutic biologic higher specific activity chinese hamster ovary also detected previously 19 associated microangiopathy saccharomyces cerevisiae </ pichia pastoris </ div >< p using mdnase1 expression recombinant murine dnase1 pastoris </ recombinant dnase1 murine dnase1 translational import since deoxyribonuclease salient feature rhdnase1 produced rhdnase1 expressed rhdnase1 due rhdnase1 ). pulmozyme ®, prolonged persistence orthologues share oligosaccharyltransferase complex native conformation methanol supply main cause lower inhibition improvements resulted impaired cleavage high structural hdnase1 resulted hdnase1 fourfold hdnase1 expression functional homology extracellular chromatin expression difference cultivation temperature concerning functionality comparative expression codon usage cell density biochemical effectivity =&# 8201 24 hours
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Sequential elution of mono- and di-N-glycosylated rmDNase1 from hydroxyapatite.

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