A. On the left, a schematic representation of the exocytosis of a VAMP7-pHluorin-positive vesicle. Once the vesicle starts to fuse with the plasma membrane, the pH becomes neutral and the pHluorin is no longer quenched and thus emits a fluorescence signal. On the right, a representative TIRFM image of VAMP7-pHluorin in a transfected HeLa cell. B. On the left, a schematic representation of the exocytosis of a CD63-pHluorin-positive vesicle. Once the vesicle starts to fuse with the plasma membrane, the CD63 + intraluminal vesicles are released in the extracellular medium and the pHluorin is exposed to a neutral pH and thus starts to emit a fluorescence signal. On the right, a representative TIRFM image of CD63-pHluorin-positive in a transfected RPE-1 cell seeded on ring-shaped micropattern with a diameter of 37µm. C. ExoDeepFinder performances in VAMP7-pHluorin transfected HeLa cells. D. Comparison of the exocytosis rate measured by manual detection (ground truth) an

<p>A. On the left, a schematic representation of the exocytosis of a VAMP7-pHluorin-positive vesicle. Once the vesicle starts to fuse with the plasma membrane, the pH becomes neutral and the pHluorin is no longer quenched and thus emits a fluorescence signal. On the right, a representative TIR...

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