Membrane integrity in the yqhG mutant.

Fluorescence-based quantification of membrane permeability in wild-type (WT), ΔyqhG, and complemented (ΔyqhG-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min....

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Auteur principal:	Jianhua Liao (1393228) (author)
Autres auteurs:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Publié:	2025
Sujets:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
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Résumé:	<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min. Bars represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA. *<i>P</i> < 0.05.</p>

Membrane integrity in the <i>yqhG</i> mutant.

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