A. Schematic representation of the exocytosis of a VAMP7-pHluorin-positive vesicle: the low pH of the acidic lumen quenches the fluorescence of pHluorin. During exocytosis, protons are released and pHluorin starts to emit light. An exocytosis event is followed by the 2D diffusion of VAMP7-pHluorin at the plasma membrane. B. TIRFM image of VAMP7-pHluorin in a transfected RPE-1 cell. The inset represents the field in the white square showing one exocytosis event at different time points, <i>t</i>  = 0 represents the peak of the exocytosis event. A kymograph is plotted along the dashed white line and arrowheads indicate several observed exocytosis events. C. Workflow of the data preparation for ExoDeepFinder training. On the one hand, manual annotation gives the coordinates of ‘ground truth’ exocytosis events, on the other hand, spots corresponding to docked vesicle are detected thanks to the ATLAS algorithm. Both annotations are merged to produce a multiclass segmentation map. The re

<p>A. Schematic representation of the exocytosis of a VAMP7-pHluorin-positive vesicle: the low pH of the acidic lumen quenches the fluorescence of pHluorin. During exocytosis, protons are released and pHluorin starts to emit light. An exocytosis event is followed by the 2D diffusion of VAMP7-p...

Full description