Verification of sgRNA Knockout Efficiency in HeLa cells.

<p>(A) METTL3-sgRNA, (B)ERK2-sgRNA, and the empty lentilCRISPR-v2 vector were co-transfected with two packaging helper plasmids, pSPAX2 and pMD2.G, into HEK-293T cells. After lentivirus production, the viruses were used to infect HeLa cells. qPCR was then used to detect the knockdown efficienc...

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Հիմնական հեղինակ:	Lucai Wang (22050158) (author)
Այլ հեղինակներ:	Xiangqi Qiu (22050161) (author), Lele Wang (542468) (author), Xilong Yang (20877464) (author), Mengjie Li (141854) (author), Xuyang Zhao (1951429) (author), Wenhui Zhu (3592448) (author), Lijie Lv (18554075) (author), Yunzhe Kang (22050164) (author), Lulu Yao (4640143) (author), Gaiping Zhang (106747) (author), Aijun Sun (466105) (author), Guoqing Zhuang (9905778) (author)
Հրապարակվել է:	2025
Խորագրեր:	Biochemistry Microbiology Molecular Biology Immunology Science Policy Mental Health Infectious Diseases Virology Biological Sciences not elsewhere classified providing new avenues intensified organ damage suppressing viral replication herpesvirus pseudorabies virus enzymatic active site mettl3 expression decreased div >< p mettl3 axis acts study reveals phosphorylation 6 </ sup erk2 phosphorylates mettl3 mettl3 axis site enhances mediated phosphorylation vivo </ vitro </ mettl3 ’ methyltransferase mettl3 κb pathway β production work establishes translational modifications terminal domain suppressing ifn serine 43 paradigm shift overexpressing erk2 novel regulator mapk signaling immunoregulatory activity identifies ser43 herpesvirus infection hastened mortality functional hotspot dependent crosstalk combined treatment antiviral defense
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Verification of sgRNA Knockout Efficiency in HeLa cells.

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