Histone Analysis Using Mobility- and Mass-Selected Ultraviolet Photodissociation in Tandem with Ultra-High-Resolution Mass Spectrometry

Histone Analysis Using Mobility- and Mass-Selected Ultraviolet Photodissociation in Tandem with Ultra-High-Resolution Mass Spectrometry

Ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry (UHR FT-ICR MS) for top-down proteomics has shown the potential to resolve proteoforms and splice variants, particularly those with post-translational modifications. Here, we integrate trapped ion mobility and mass sel...

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Bibliographic Details
Main Author: Cassandra N. Fuller (19474750) (author)
Other Authors: Md Shofiul Alam (19198905) (author), Kevin Jeanne Dit Fouque (1686298) (author), Lilian Valadares Tose (19474753) (author), Richard M. Searfoss (21593243) (author), Francisca N. de Luna Vitorino (21593246) (author), Mariangela Kosmopoulou (13813759) (author), Detlev Suckau (2305423) (author), Mark E. Ridgeway (1308147) (author), Steve Van Orden (4690837) (author), Benjamin A. Garcia (205670) (author), Francisco Fernandez-Lima (761320) (author)
Published: 2025
Subjects:
Biochemistry
Cell Biology
Genetics
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
minimal sample preparation
h4 core histones
confirmed using bottom
bovine histone mixture
better duty cycle
icr ms measurements
icr ms allows
high sequence coverages
selected ultraviolet photodissociation
icr ms
ultraviolet photodissociation
histone variants
selected uvpd
work provides
translational modifications
splice variants
spectral decongestion
resolve proteoforms
ptm localization
ptm combinations
protein mixtures
precursor ions
ions led
including acetylation
general applicability
effective mobility
better annotation
77 %).
51 proteoforms
2 ×).
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