Generation of recombinant KSHV BAC with different numbers of TR copies.

(A). Schematic diagram of KSHV BAC16 with different copy numbers of TR. The kanamycin cassette with I-SceI recognition sequence, along with the TR homologous sequence, was generated by PCR with pEP-Kan plasmid as a template. This DNA...

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Hlavní autor:	Tomoki Inagaki (12903779) (author)
Další autoři:	Ashish Kumar (152984) (author), Kang-Hsin Wang (12691954) (author), Somayeh Komaki (17350755) (author), Jonna M. Espera (22784197) (author), Christopher S. A. Bautista (22784200) (author), Ken-ichi Nakajima (622199) (author), Chie Izumiya (449437) (author), Yoshihiro Izumiya (449433) (author)
Vydáno:	2025
Témata:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Developmental Biology Cancer Science Policy Infectious Diseases Virology specific genomic region de novo </ shortened dna template ef1α promoter encoded infected kshv episomes generated recombinant kshv viral gene promoters tr copy numbers tr copy number intrinsically disordered domain inducible lana degradation containing protein 4 inducible promoter activation tr dna fragments brd4 interacts flexibly supported kshv reactivation kshv tr functions previous studies demonstrated div >< p smaller lana nbs studied brd4 recruitment larger lana nbs prior transcription activation reactivating cells confirmed prior activation promoter dynamics larger brd4 dna bindings brd4 recruitment tr length impaired reactivation containing llps gene enhancer enhancer domain brd4 occupancies transcription memories transcription factor terminal repeats tagged proteins separated dots results suggest related proteins purified fluorescence liquid phase kaposi ’ global attenuation genome contains form liquid either due decreased efficacy
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Generation of recombinant KSHV BAC with different numbers of TR copies.

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