MAb binding to JR-FL gp160ΔCT E168K+N189A in BN-PAGE “shifts”.

<p>MAbs were mixed with SOS VLP (A and B) or SOS NFL I559P VLP (C and D) and were incubated at 37°C for 1h, then washed, lysed and analyzed by BN-PAGE-Western blot, probing with anti-gp120+gp41 MAb cocktail, followed by anti-human IgG AP conjugate (A and C) or just the anti-human IgG AP conjug...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Tommy Tong (426980) (author)
مؤلفون آخرون:	Alessio D’Addabbo (11597230) (author), Jiamin Xu (5613173) (author), Himanshi Chawla (10522421) (author), Albert Nguyen (2580886) (author), Paola Ochoa (16793086) (author), Max Crispin (346213) (author), James M. Binley (8026892) (author)
منشور في:	2023
الموضوعات:	Biophysics Biochemistry Immunology Infectious Diseases Virology soluble envelope glycoprotein native protein gels interface nab binding impacted v2 apex high mannose gp160 findings suggest caution artificially stabilized laterally 1 vaccine development show glycan skipping supporting antigenic differences increased sequon skipping reduced glycan maturation helix mutations i559p glycan processing enzymes markedly reduced gp120 also reduced gp120 common side effects leveraging stabilizing mutations stabilize membrane trimers glycan maturation stabilizing mutations gp41 processing antigenic profile increased rigidity greatly increased viral gp120 inefficient gp120 expression gp120 used virus nucleic acid neutralizing epitopes monoclonal antibodies minor species limits access like particle human plasmas gp41 n gp41 ). fusion conformation exposed non based immunogens 293t cells &# 8220
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MAb binding to JR-FL gp160ΔCT E168K+N189A in BN-PAGE “shifts”.

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