RNG2 spans between the APR and the conoid, forming 22 tethers.
<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)<...
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2025
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| Sumario: | <p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)</b> Protein depletion after 48 h of auxin (IAA) treatment assessed by western blot. <b>(C)</b> Western blot of parasite lysate collected in intracellular or extracellular state <b>(D)</b> U-ExM image of the locations of RNG2-mAID depending on the tag insertion and RNG2 polyclonal antibody (recognizing the central region). Images were taken with extruded and retracted conoids. Scale bar = 3 μm. <b>(E)</b> Iterative expansion microscopy (iU-ExM) of the N-terminal (Myc), middle (Ty), and C-terminal (HA) tags of RNG2-mAID. Scale bar = 3 μm. Right panel: Distribution of the numbers of dots counted for the anti-HA staining across 13 images. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(F) Left panel:</b> Electron microscopy images of the <i>T</i>oxoplasma <i>gondii</i> apical complex as an ultrastructure control. <b>Right panel:</b> Immunoelectron microscopy images obtained with the α-GAC and α-RNG2 antibody. Red arrows = gold particles. Scale bar = 100 nm.</p> |
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