RNG2 spans between the APR and the conoid, forming 22 tethers.

RNG2 spans between the APR and the conoid, forming 22 tethers.

<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)<...

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में बचाया:

ग्रंथसूची विवरण
मुख्य लेखक:	Romuald Haase (22339777) (author)
अन्य लेखक:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
प्रकाशित:	2025
विषय:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
टैग:	टैग जोड़ें कोई टैग नहीं, इस रिकॉर्ड को टैग करने वाले पहले व्यक्ति बनें!

समान संसाधन

Model RNG2 as a molecular tether for conoid apical anchorage.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Phenotypic consequences of the conoid detachment.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

RNG2 is functional only as intact, full-length protein.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Investigation of RNG2 subdomain functions.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Biochemical investigation of wild-type and processed RNG2.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

<i>Toxoplasma gondii</i> tachyzoite cell organization.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Reconstruction of the Fig 4E panel III.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Reconstruction of the Fig 4E panel II.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Reconstruction of the Fig 4E panel I.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Source Numerical Data of this study.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Additional information on antibodies and primers used in this study.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Additional information on the RNG2 variants expressed in UPRT locus.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Additional information on the cryo-electron tomography performed on RNG2-mAID strain.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Additional information on the processed forms and localization of the RNG2 protein.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Multiple epitopes-tagging across RNG2.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Additional information of the RNG2 purification from insect cells.
द्वारा: Romuald Haase (22339777)
प्रकाशित: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
द्वारा: Pengchao Zheng (22674589)
प्रकाशित: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
द्वारा: Pengchao Zheng (22674589)
प्रकाशित: (2025)

A. Mouse transcriptome non-negative matrix factorization component weights across all time points and conditions. Larger weight values indicate components with upregulated genes dominating. B. Gene set enrichment plot for selected features from each component in panel A. Components 1, 4, and 5 did not exhibit enrichment in these broad terms. C. Number of genes in enriched gene ontology terms related to immunity, signaling, and transcription for each time point and condition. D. Time course transcriptional profiles for mouse genes characteristic to component 3. E. PARFA-seq validation by quantitative PCR and gene expression changes during immortalized bone marrow-derived macrophage infection by <i>T. gondii</i> assessed by quantitative PCR.
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

A. <i>T. gondii</i> gene group changes throughout the time course, expressed as log<sub>2</sub>(fold change) relative to pre-infection tachyzoites (t = 0). Grey area depicts the 95% confidence interval. n value refers to number of genes detected in each group. B. Gene set enrichment plot for most enriched terms from <i>T. gondii</i> transcriptome features at 15 min to 120 min. Five most significant terms are displayed for each database. C and D. Transcriptional profiles of top five upregulated and downregulated <i>T. gondii</i> transcription factors, respectively.
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

A. Library preparation strategy: poly-A(+) RNAs are captured by complementarity to reverse transcription primer, then second strand is synthesized using random hexamers and handles for sequencing are attached through two rounds of PCR. B. Mapped read distribution by species and by biotype. C and D. Principal component analysis of mouse and <i>T. gondii</i> transcriptomes, respectively. E and F. Volcano plots of Live <i>Toxoplasma</i>-infected mouse and <i>T. gondii</i> time points, respectively. To calculate Log<sub>2</sub>(Fold change), every timepoint is compared to corresponding samples (either macrophage or <i>Toxoplasma</i>) before infection (t = 0 min). Most significant and most upregulated transcripts are labelled. Number of significantly (p ≤ 0.05) downregulated and upregulated genes for each time point is given in blue and red, respectively. In F, 24-hour time point, red dots mark ROP
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

A. Experimental scheme of infection time course and downstream workflow; B. Optimization of tachyzoite concentration to achieve adequate infection rates, with a threshold set at 50% infection efficiency. Error bars represent ± 1 standard deviation. Corresponding MOI values are given for each condition; C. Infection time course micrograph merged from brightfield and green channels. <i>T. gondii</i> exhibits green fluorescence due to stable GFP expression in the strain used. Percentage at the top left indicates infection efficiency (± 1 standard deviation) for each time point.
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

Summary of all significant gene enrichment terms for every condition (as combination of sample type and timepoint).
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

Oligonucleotides used in this study.
द्वारा: Dominykas Murza (22676289)
प्रकाशित: (2025)

<b>No effect of actual parasitism risk on egg rejection in two sympatric breeding cuckoo hosts</b>
द्वारा: Hanlin Yan (19840763)
प्रकाशित: (2025)

Transcriptomic insights into the tripartite plant–pathogen–mycoparasite interaction reveal novel mechanisms involving fungal secretomes and plant amino acid metabolism
द्वारा: kazuya maeda (17387962)
प्रकाशित: (2025)

Table 3_Harnessing mRNA technology against Fasciola hepatica: Immunological insights from a fatty acid binding protein vaccine.docx
द्वारा: Javier Sánchez-Montejo (22681769)
प्रकाशित: (2025)

Data Sheet 1_Harnessing mRNA technology against Fasciola hepatica: Immunological insights from a fatty acid binding protein vaccine.docx
द्वारा: Javier Sánchez-Montejo (22681769)
प्रकाशित: (2025)

Table 2_Harnessing mRNA technology against Fasciola hepatica: Immunological insights from a fatty acid binding protein vaccine.docx
द्वारा: Javier Sánchez-Montejo (22681769)
प्रकाशित: (2025)

Table 1_Harnessing mRNA technology against Fasciola hepatica: Immunological insights from a fatty acid binding protein vaccine.docx
द्वारा: Javier Sánchez-Montejo (22681769)
प्रकाशित: (2025)

Data Sheet 1_Innovations in invasive parasite control: enhancing nest treatment techniques to combat the threat of the avian vampire fly Philornis downsi in Galapagos.docx
द्वारा: Barbara Kofler (108878)
प्रकाशित: (2025)

Supplementary Data 1 from Targeting BCL2 Overcomes Resistance and Augments Response to Aurora Kinase B Inhibition by AZD2811 in Small Cell Lung Cancer
द्वारा: Kavya Ramkumar (16474203)
प्रकाशित: (2025)

Summary of the general phenotype of SH3 domain-containing gene mutants.
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

SH3 domain-containing genes and the subcellular localization of their respective products in <i>F. graminearum.</i>
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

The IDRs in SH3 domain-containing proteins are important for the fusion of cytoplasmic protein condensates.
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

SH3 domain-containing proteins exhibit features of phase separation in <i>F. graminearum.</i>
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

Effects of <i>FgHOF1</i> deletion on cytoskeletal and nuclear distribution.
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

Co-localization of FgAip5-GFP at hyphal tips and various SH3-domain proteins at membrane-associated structures.
द्वारा: Yakubu Saddeeq Abubakar (5558399)
प्रकाशित: (2025)

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