Comparison between Cq and copy number determined using droplet digital PCR amplification.

<p>A. The qPCR Cq values and ddPCR copy number collected for all sample types, for RNR and WG target amplification, respectively (black dots, n = 251). Regression curve fit to data, plotted as solid blue line (R<sup>2</sup> = 0.85748). B. Residuals of regression in panel A with res...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Nathaniel Ponvert (17184784) (author)
مؤلفون آخرون:	Frank Byrne (21354976) (author), Monique J. Rivera (17204545) (author), Timo Rohula (21354979) (author), Sandra Olkowski (2137909) (author), Heshani De Silva Weligodage (21354982) (author), Neil McRoberts (4716426) (author), Judith K. Brown (11722838) (author)
منشور في:	2025
الموضوعات:	Microbiology Biotechnology Ecology Sociology Plant Biology Biological Sciences not elsewhere classified vector diseases affecting time quantitative pcr ten per tube psyllid developmental stage droplet digital pcr california using real 4 – 5 liberibacter asiaticus ’ genome load peaking div >< p asian citrus psyllid diaphorina citri </ california citrus groves adult acp might adult acp age citrus greening disease las detection correlated consistent qpcr detection citrus groves adult acp grove ’ genome copies citrus trees citrus industry >< b candidatus </ c </ acp adults temporal detection reliable detection las detection early detection detection rates uneven distribution symptomatic phase stable throughout sample size results indicated qpcr amplification potentially misrepresenting optimized scouting optimize approaches optimal number long pre las throughout las status infected trees field populations dna isolation destructive bacterial
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Comparison between Cq and copy number determined using droplet digital PCR amplification.

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