Measuring increased metabolism with sub-MIC antibiotic treatment.

a) Reduction of the tetrazolium salt MTT into a formazan dye by cellular metabolism. b) Images of the zone of inhibition on the MTT agar plates for TET, CEF, and GENT that were used for quantification. c) Pixel intensity as a function...

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Bibliographic Details
Main Author:	Luke N. Yaeger (17328437) (author)
Other Authors:	Shawn French (1444561) (author), Eric D. Brown (751415) (author), Jean Philippe Côté (17328440) (author), Lori L. Burrows (394970) (author)
Published:	2023
Subjects:	Microbiology Genetics Physiology Ecology Science Policy Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified vehicle control conditions showed spatial co poorly characterized mechanisms increased metabolic activity identify core components identified multiple avenues gene encoding part div >< p alternative electron acceptor activated ’ phenotype biofilm matrix production respiring nitrate abolished implicating central metabolism already high relative escherichia coli </ bactericidal antibiotics cefixime biofilm stimulation response nitrate respiration increased k12 deletion mutants central metabolism stimulation response biofilm stimulation coli </ mic antibiotics biofilm formation nitrate supplementation vivo </ nuoe </ lpda </ e </ arcb </ acna </ ‘ pre wild type translation inhibitors tetrazolium dye systems level sensitive two respiratory stress nadh sensor mutants lacking minimally affected mic cefixime lethal concentrations key player distinct ways component system antibiotic classes ameliorating effects aerobic respiration
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Measuring increased metabolism with sub-MIC antibiotic treatment.

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