Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models
<p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated...
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2025
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| 总结: | <p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated cells from the experiment described in <b>A</b>, at 16 hours after IR. <b>C,</b> Micronuclei in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 48 hours after IR. Data points indicate the mean ± SD from triplicate wells and graphs are representative from three separate experiments. <b>D,</b> Representative images from irradiated cells from the experiment described in <b>C</b>. Micronuclei are indicated with arrow heads. dsDNA, double-stranded DNA. <b>E,</b> RAD51 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 6 hours after IR. <b>F,</b> Representative images from irradiated cells from the experiment described in <b>E</b>. Lines and error bars in <b>A</b> and <b>E</b> correspond to average ± SD representative from three independent experiments, and statistical significance was calculated using nonparametric one-way ANOVA (Kruskal–Wallis) with Dunn correction for multiple comparisons (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001).</p> |
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