Validation of Dcr-2 knockout in the Aag2 319 cell line.

<p>The parental Aag2 AF5 cell line and the Dcr-2 k.o. cell line Aag2 AF319 were co-transfected with a Rluc expressing construct (pIZ-Rluc) and dsRNA against the Rluc gene (dsRluc). Co-transfection of dsRNA against the eGFP gene (dsGFP) was used as control. At 24 h post transfection cells were...

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المؤلف الرئيسي:	Anja vom Hemdt (19787189) (author)
مؤلفون آخرون:	Alexandra L. Thienel (19787192) (author), Katrin Ciupka (14712151) (author), Janett Wieseler (19787195) (author), Hannah M. Proksch (19787198) (author), Martin Schlee (10672) (author), Beate M. Kümmerer (17748614) (author)
منشور في:	2024
الموضوعات:	Microbiology Cell Biology Molecular Biology Immunology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified several mutations across largely diminished dissemination div >< p aedes albopictus </ aedes aegypti </ >- derived c6 viral growth analyses analyses using chimeras viral genome resulting viral genome accompanied type asibi strain structural protein ns5 rna sensor rig various mosquito cells mosquito cells revealed asibi cap0 attenuation secondary tissues compared unmethylated cap0 structure asibi cap1 correlates lower infection rates yfv cap0 viruses 17d cap0 replication mosquito cells cap0 viruses asibi cap0 secondary tissues rna genome cap0 structure asibi cap1 cap1 structure replication analyses yfv ns5 asibi suggest 36 cells 17d genome oral infection lower extent intrathoracic infection vaccine 17d synergistic effect striking difference results suggest restricted replication orthoflaviviruses encodes inhibition differed entirely dispensable distinct downregulation decreased amounts completely blocked analyzed whether also suppressed
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Validation of Dcr-2 knockout in the Aag2 319 cell line.

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