Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff
<p>Macrophages (MØs) are pivotal immune cells exhibiting significant plasticity that has been widely studied in human and murine models. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) are key regulators of macrophage differentiation f...
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2025
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| author | Yanina P. Hecker (22679417) |
| author2 | Montserrat Coronado (16545327) Clara Hurtado-Morillas (21507251) David Arranz-Solís (3555875) Roberto Sánchez-Sánchez (4751217) Ángel Corbí (2580331) Luis Miguel Ortega-Mora (6172994) |
| author2_role | author author author author author author |
| author_facet | Yanina P. Hecker (22679417) Montserrat Coronado (16545327) Clara Hurtado-Morillas (21507251) David Arranz-Solís (3555875) Roberto Sánchez-Sánchez (4751217) Ángel Corbí (2580331) Luis Miguel Ortega-Mora (6172994) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yanina P. Hecker (22679417) Montserrat Coronado (16545327) Clara Hurtado-Morillas (21507251) David Arranz-Solís (3555875) Roberto Sánchez-Sánchez (4751217) Ángel Corbí (2580331) Luis Miguel Ortega-Mora (6172994) |
| dc.date.none.fl_str_mv | 2025-11-25T06:26:17Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2025.1680086.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image_1_Ovine_macrophage_identity_and_plasticity_novel_insights_into_CSF-driven_polarization_and_species-specific_responses_tiff/30704237 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Genetic Immunology ovine macrophages GM-CSF M-CSF immunophenotype RNAseq |
| dc.title.none.fl_str_mv | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Macrophages (MØs) are pivotal immune cells exhibiting significant plasticity that has been widely studied in human and murine models. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) are key regulators of macrophage differentiation from monocytes. In this study, we comprehensively investigated the immunophenotypic, functional, and transcriptomic profiles of ovine MØs differentiated with GM-CSF (GM-oMØs) or M-CSF (M-oMØs) to provide a more nuanced understanding of their activation states. After 7 days, GM-oMØs displayed a smaller, more varied morphology with lower cell yields compared to the larger, uniformly amoeboid M-oMØs. Immunophenotypically, M-oMØs showed significantly higher CD163 expression, consistent with human M-MØs, while CLEC5A was uninformative for differentiation. Transcriptomic analysis, complemented by qPCR and ELISA, revealed clearly distinct profiles, with GM-oMØs exhibiting a pronounced pro-inflammatory phenotype and showing significantly higher expression of 408 genes, mostly associated with interferon and inflammatory response pathways, a feature that aligns with the functional and phenotypic characteristics of human and mouse GM-MØ. Conversely, M-oMØs displayed a regulatory and anti-inflammatory profile, marked by a significantly higher expression of IL-10 and a set of 248 genes involved in cellular homeostasis. Notably, LPS stimulation dramatically shifted the M-oMØ phenotype toward a pro-inflammatory state, unequivocally demonstrating their substantial plasticity, and mirroring human M-CSF-polarized monocytes. Our findings fundamentally challenge the prevailing M1/M2 simplification in ovine macrophage biology and provide a robust foundation for selecting appropriate in vitro macrophage models for future investigations into ovine host defense and disease pathogenesis. This study demonstrated that M-oMØs exhibit greater plasticity, making them more suitable for pathogen-host interaction studies. Unlike GM macrophages, which already have a defined phenotype, M-oMØs more accurately reflect the dynamic immune response induced by a pathogen in the host.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_2f9a353fb9fa22d2ac4f7bf245dd6890 |
| identifier_str_mv | 10.3389/fimmu.2025.1680086.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30704237 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiffYanina P. Hecker (22679417)Montserrat Coronado (16545327)Clara Hurtado-Morillas (21507251)David Arranz-Solís (3555875)Roberto Sánchez-Sánchez (4751217)Ángel Corbí (2580331)Luis Miguel Ortega-Mora (6172994)Genetic Immunologyovine macrophagesGM-CSFM-CSFimmunophenotypeRNAseq<p>Macrophages (MØs) are pivotal immune cells exhibiting significant plasticity that has been widely studied in human and murine models. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) are key regulators of macrophage differentiation from monocytes. In this study, we comprehensively investigated the immunophenotypic, functional, and transcriptomic profiles of ovine MØs differentiated with GM-CSF (GM-oMØs) or M-CSF (M-oMØs) to provide a more nuanced understanding of their activation states. After 7 days, GM-oMØs displayed a smaller, more varied morphology with lower cell yields compared to the larger, uniformly amoeboid M-oMØs. Immunophenotypically, M-oMØs showed significantly higher CD163 expression, consistent with human M-MØs, while CLEC5A was uninformative for differentiation. Transcriptomic analysis, complemented by qPCR and ELISA, revealed clearly distinct profiles, with GM-oMØs exhibiting a pronounced pro-inflammatory phenotype and showing significantly higher expression of 408 genes, mostly associated with interferon and inflammatory response pathways, a feature that aligns with the functional and phenotypic characteristics of human and mouse GM-MØ. Conversely, M-oMØs displayed a regulatory and anti-inflammatory profile, marked by a significantly higher expression of IL-10 and a set of 248 genes involved in cellular homeostasis. Notably, LPS stimulation dramatically shifted the M-oMØ phenotype toward a pro-inflammatory state, unequivocally demonstrating their substantial plasticity, and mirroring human M-CSF-polarized monocytes. Our findings fundamentally challenge the prevailing M1/M2 simplification in ovine macrophage biology and provide a robust foundation for selecting appropriate in vitro macrophage models for future investigations into ovine host defense and disease pathogenesis. This study demonstrated that M-oMØs exhibit greater plasticity, making them more suitable for pathogen-host interaction studies. Unlike GM macrophages, which already have a defined phenotype, M-oMØs more accurately reflect the dynamic immune response induced by a pathogen in the host.</p>2025-11-25T06:26:17ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fimmu.2025.1680086.s001https://figshare.com/articles/figure/Image_1_Ovine_macrophage_identity_and_plasticity_novel_insights_into_CSF-driven_polarization_and_species-specific_responses_tiff/30704237CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307042372025-11-25T06:26:17Z |
| spellingShingle | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff Yanina P. Hecker (22679417) Genetic Immunology ovine macrophages GM-CSF M-CSF immunophenotype RNAseq |
| status_str | publishedVersion |
| title | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| title_full | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| title_fullStr | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| title_full_unstemmed | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| title_short | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| title_sort | Image 1_Ovine macrophage identity and plasticity: novel insights into CSF-driven polarization and species-specific responses.tiff |
| topic | Genetic Immunology ovine macrophages GM-CSF M-CSF immunophenotype RNAseq |