Engineering a New SsrA-Based Degradation Tag (LAA-LAA) and a Bacterial Synthetic Oscillator

The ATP-dependent ClpXP-SspB protease complex is responsible for the degradation of intracellular proteins and is maintained at low levels in Escherichia coli to avoid nonspecific degradation. The rate-limiting step in the protease complex leads to proteolytic queueing, where the proteins form waiti...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Prajakta Jadhav (8218539) (author)
مؤلفون آخرون:	Sudeshna Roy (1340793) (author), Xuan Yi Butzin (20902636) (author), Nicholas C. Butzin (8218545) (author)
منشور في:	2025
الموضوعات:	Biochemistry Microbiology Genetics Molecular Biology Physiology Pharmacology Developmental Biology Infectious Diseases Biological Sciences not elsewhere classified Information Systems not elsewhere classified steady oscillation period independent chaperone ). entrainment across 1000s avoid nonspecific degradation original oscillator ’ chaperone binding sequences osc oscillator retains amino acid clpx bacterial synthetic oscillator protease complex leads new oscillator could tagged protein levels overall degradation rate leveraged proteolytic queueing original ssra tag precise component responsible based degradation tag sspb protease complex original oscillator osc oscillator dependent chaperone ssra tag feedback oscillator proteolytic queueing low levels entrainment using binding site dependent tag synthetic circuit synthetic biologists ssra variants sspb complex new ssra laa tag vivo </ upon overexpression tagging proteins sspb system previous work old oscillators limiting step intracellular proteins increased amount external signal evidence supports escherichia coli distinct frequencies different frequency component affects characterized dual best characteristics
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Engineering a New SsrA-Based Degradation Tag (LAA-LAA) and a Bacterial Synthetic Oscillator

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