Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations.
<p><b>A)</b> Optogenetic stimulation timings for entire and early window conditions aligned to stimulus onset (STIM ON) or offset (STIM OFF) (top), schematic of optogenetic method combined with 2-photon imaging (middle), schematic of laser activating only ChrimsonR-expressing PV ce...
সংরক্ষণ করুন:
| প্রধান লেখক: | |
|---|---|
| অন্যান্য লেখক: | , , , |
| প্রকাশিত: |
2025
|
| বিষয়গুলি: | |
| ট্যাগগুলো: |
ট্যাগ যুক্ত করুন
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
|
| _version_ | 1849927627015454720 |
|---|---|
| author | Lilia Kukovska (22683586) |
| author2 | Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| author2_role | author author author author |
| author_facet | Lilia Kukovska (22683586) Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| author_role | author |
| dc.creator.none.fl_str_mv | Lilia Kukovska (22683586) Katharina A. Wilmes (22683589) Natsumi Y. Homma (22683592) Claudia Clopath (324706) Jasper Poort (22683595) |
| dc.date.none.fl_str_mv | 2025-11-25T18:37:28Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003518.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Changes_in_neural_selectivity_caused_by_PV_cell_activation_reflect_behavioral_performance_in_easy_but_not_difficult_discriminations_/30714571 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |
| dc.title.none.fl_str_mv | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>A)</b> Optogenetic stimulation timings for entire and early window conditions aligned to stimulus onset (STIM ON) or offset (STIM OFF) (top), schematic of optogenetic method combined with 2-photon imaging (middle), schematic of laser activating only ChrimsonR-expressing PV cells while GCaMP7s reports the activity of all neurons (bottom). <b>B)</b> Example frames of in-vivo imaging plane showing GCaMP7s-expressing neurons when illuminated with 920 nm wavelength (left), and ChrimsonR-tdTomato-expressing neurons when illuminated with 1,040 nm wavelength (right). Arrow shows example PV cell. Scale bar, 50 µm. <b>C)</b> Average response of two example neurons to optogenetic laser illumination during the entire stimulus duration (0–2 s). The activity to the visual stimulus of the identified Pyr cell is suppressed, whereas the PV cell is increased. Shading, SEM. <b>D)</b> Median laser-induced changes in absolute selectivity for entire (left panel, <i>N</i> = 1,156 cells, 6 mice) and early (right panel, <i>N</i> = 754 cells, 6 mice) time window conditions, for easy task, significant at all laser levels (Wilcoxon signed-rank test, <i>p</i> < 0.002). Error bars, SEM. Laser levels in D–G) are calibrated for each mouse to cause moderate suppression in Pyr cell activity (see Methods) and range from 0.02 to 0.37 mW. <b>E)</b> is same as D) showing entire (left panel, <i>N</i> = 542 cells, 3 mice) and early (right panel, <i>N</i> = 561 cells, 3 mice) time window conditions but for difficult task (Wilcoxon signed-rank test, ‘entire’ laser level 1 = −0.03 ± 0.01, <i>p</i> = 0.017; ‘early’ laser level 2 = 0.01 ± 0.01, <i>p</i> = 0.037, all other laser levels n.s.). <b>F)</b> Stimulus selectivity index of the same cells as in D) in the no laser condition (0 mW, off) and across laser levels for easy task. Cells were ordered by their selectivity at each laser level. <b>G)</b> is same as F) but for difficult task. Color bar range indicates selectivity with positive values for cells preferring the rewarded ‘go’ stimulus and negative values for cells preferring the unrewarded ‘no-go’ stimulus. <b>H)</b> Histograms of data in F) to quantify the shift towards go or no-go preference in the easy task. Entire: Median ± SEM, laser off = 0.16 ± 0.02, laser on = 0.18 ± 0.02, Wilcoxon signed-rank test, <i>p</i> < 0.002; averaged across laser levels. <b>I)</b> Histograms of data in G) to quantify the shift towards go or no-go preference in the difficult task. Entire: Median ± SEM, laser off = 0.15 ± 0.02, laser on = 0.12 ± 0.01, Wilcoxon signed-rank test, <i>p</i> = 0.931; averaged across laser levels. Early: Median ± SEM, laser off = 0.15 ± 0.01, laser on = 0.10 ± 0.01, Wilcoxon signed-rank test, <i>p</i> < 0.002; averaged across laser levels.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_37ad99f6e2872ed6d2a58ded5fc93a4f |
| identifier_str_mv | 10.1371/journal.pbio.3003518.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30714571 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations.Lilia Kukovska (22683586)Katharina A. Wilmes (22683589)Natsumi Y. Homma (22683592)Claudia Clopath (324706)Jasper Poort (22683595)BiochemistryNeuroscienceDevelopmental BiologyScience PolicyMental HealthBiological Sciences not elsewhere classifiedxlink "> inhibitionthree major factorsstrong competitive interactionsphoton imaging showedbalanced cortical activityprimary visual cortexsustained v1 activitycombined optogenetic activationv1 cells capturedmoderate pv activationmoderate activationvisual discriminationsexpressing cellsnonlinear activationdependent activationactivation strengthtask difficultystimulus onsetperceptual discriminationdifficult discriminationsdelineates conditionscircuit modelbehavioral effects<p><b>A)</b> Optogenetic stimulation timings for entire and early window conditions aligned to stimulus onset (STIM ON) or offset (STIM OFF) (top), schematic of optogenetic method combined with 2-photon imaging (middle), schematic of laser activating only ChrimsonR-expressing PV cells while GCaMP7s reports the activity of all neurons (bottom). <b>B)</b> Example frames of in-vivo imaging plane showing GCaMP7s-expressing neurons when illuminated with 920 nm wavelength (left), and ChrimsonR-tdTomato-expressing neurons when illuminated with 1,040 nm wavelength (right). Arrow shows example PV cell. Scale bar, 50 µm. <b>C)</b> Average response of two example neurons to optogenetic laser illumination during the entire stimulus duration (0–2 s). The activity to the visual stimulus of the identified Pyr cell is suppressed, whereas the PV cell is increased. Shading, SEM. <b>D)</b> Median laser-induced changes in absolute selectivity for entire (left panel, <i>N</i> = 1,156 cells, 6 mice) and early (right panel, <i>N</i> = 754 cells, 6 mice) time window conditions, for easy task, significant at all laser levels (Wilcoxon signed-rank test, <i>p</i> < 0.002). Error bars, SEM. Laser levels in D–G) are calibrated for each mouse to cause moderate suppression in Pyr cell activity (see Methods) and range from 0.02 to 0.37 mW. <b>E)</b> is same as D) showing entire (left panel, <i>N</i> = 542 cells, 3 mice) and early (right panel, <i>N</i> = 561 cells, 3 mice) time window conditions but for difficult task (Wilcoxon signed-rank test, ‘entire’ laser level 1 = −0.03 ± 0.01, <i>p</i> = 0.017; ‘early’ laser level 2 = 0.01 ± 0.01, <i>p</i> = 0.037, all other laser levels n.s.). <b>F)</b> Stimulus selectivity index of the same cells as in D) in the no laser condition (0 mW, off) and across laser levels for easy task. Cells were ordered by their selectivity at each laser level. <b>G)</b> is same as F) but for difficult task. Color bar range indicates selectivity with positive values for cells preferring the rewarded ‘go’ stimulus and negative values for cells preferring the unrewarded ‘no-go’ stimulus. <b>H)</b> Histograms of data in F) to quantify the shift towards go or no-go preference in the easy task. Entire: Median ± SEM, laser off = 0.16 ± 0.02, laser on = 0.18 ± 0.02, Wilcoxon signed-rank test, <i>p</i> < 0.002; averaged across laser levels. <b>I)</b> Histograms of data in G) to quantify the shift towards go or no-go preference in the difficult task. Entire: Median ± SEM, laser off = 0.15 ± 0.02, laser on = 0.12 ± 0.01, Wilcoxon signed-rank test, <i>p</i> = 0.931; averaged across laser levels. Early: Median ± SEM, laser off = 0.15 ± 0.01, laser on = 0.10 ± 0.01, Wilcoxon signed-rank test, <i>p</i> < 0.002; averaged across laser levels.</p>2025-11-25T18:37:28ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003518.g005https://figshare.com/articles/figure/Changes_in_neural_selectivity_caused_by_PV_cell_activation_reflect_behavioral_performance_in_easy_but_not_difficult_discriminations_/30714571CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307145712025-11-25T18:37:28Z |
| spellingShingle | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. Lilia Kukovska (22683586) Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |
| status_str | publishedVersion |
| title | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| title_full | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| title_fullStr | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| title_full_unstemmed | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| title_short | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| title_sort | Changes in neural selectivity caused by PV cell activation reflect behavioral performance in easy but not difficult discriminations. |
| topic | Biochemistry Neuroscience Developmental Biology Science Policy Mental Health Biological Sciences not elsewhere classified xlink "> inhibition three major factors strong competitive interactions photon imaging showed balanced cortical activity primary visual cortex sustained v1 activity combined optogenetic activation v1 cells captured moderate pv activation moderate activation visual discriminations expressing cells nonlinear activation dependent activation activation strength task difficulty stimulus onset perceptual discrimination difficult discriminations delineates conditions circuit model behavioral effects |