Additional information on the processed forms and localization of the RNG2 protein.
<p><b>(A)</b> Mapping of the processed formed of RNG2 observed across a variety of western blot in RNG2 variants expressed in insect cell and the endogenous RNG2 in <i>Toxoplasma gondii.</i> Five main cleavage sites are identified shared between Sf9 cells and <i>T...
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2025
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| _version_ | 1849927642255458304 |
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| author | Romuald Haase (22339777) |
| author2 | Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author2_role | author author author author author author author author author author |
| author_facet | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author_role | author |
| dc.creator.none.fl_str_mv | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| dc.date.none.fl_str_mv | 2025-11-24T18:30:50Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003506.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Additional_information_on_the_processed_forms_and_localization_of_the_RNG2_protein_/30697317 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| dc.title.none.fl_str_mv | Additional information on the processed forms and localization of the RNG2 protein. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Mapping of the processed formed of RNG2 observed across a variety of western blot in RNG2 variants expressed in insect cell and the endogenous RNG2 in <i>Toxoplasma gondii.</i> Five main cleavage sites are identified shared between Sf9 cells and <i>T. gondii</i>. <b>(B)</b> Western blot analysis of a solubility assay revealed using the different tags inserted into the RNG2-mAID strain. Catalase is a solubility control soluble in PBS and IMC1 is an insoluble control soluble only in 1% SDS. <b>(C)</b> Colocalization of the Ty-Tag and αRNG2 antibody with the N-terminal (Myc) or C-terminal (HA) tags performed by U-ExM with extruded conoid. Scale bar = 3 μm. Plot profile analysis of the signal. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(D)</b> Colocalization of the Ty-Tag with the N-terminal (Myc) or C-terminal (HA) tags performed by U-ExM with retracted conoid. Scale bar = 3 μm. Plot profile analysis of the signal. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_3cfb4b5dc34091660fb99ac31cab1b64 |
| identifier_str_mv | 10.1371/journal.pbio.3003506.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697317 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Additional information on the processed forms and localization of the RNG2 protein.Romuald Haase (22339777)Bingjian Ren (22676577)Albert Tell i Puig (22676580)Alessandro Bonavoglia (19415718)Jean-Baptiste Marq (375239)Rémy Visentin (22676583)Nicolas Dos Santos Pacheco (22676586)Bohumil Maco (383443)Ricardo Mondragón-Flores (10737057)Oscar Vadas (2416228)Dominique Soldati-Favre (79026)BiophysicsBiochemistryMicrobiologyCell BiologyPhysiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedtoxoplasma gondii </remains apically anchoredheterologous expression systemsdiv >< papical polar ringbiochemical analysis revealedproper conoid anchorageensures conoid functionalityless parasites revealedhost cell invasionenable parasite motilitycontrolling parasite motilityalthough rng2 depletionintact conoid organelleorganelle traversesintact proteinconditional depletionbiochemical propertiesdetached conoidgliding motilityunstable proteinundergoes extrusiontwo structuresstrong candidatestriking detachmentstill unclearrng2 tethersrng2 ledrng2 emergesrhoptries followresilient bridgeprotein localizedplasma membranepivotal proteinlikely facilitatelarge coiledimmunoelectron microscopyfunctional activityenabling rng2electron tomographydependent extrusioncritical roleconcatenated assembliescomprehensive mutagenesiscoil domainscalcium waves<p><b>(A)</b> Mapping of the processed formed of RNG2 observed across a variety of western blot in RNG2 variants expressed in insect cell and the endogenous RNG2 in <i>Toxoplasma gondii.</i> Five main cleavage sites are identified shared between Sf9 cells and <i>T. gondii</i>. <b>(B)</b> Western blot analysis of a solubility assay revealed using the different tags inserted into the RNG2-mAID strain. Catalase is a solubility control soluble in PBS and IMC1 is an insoluble control soluble only in 1% SDS. <b>(C)</b> Colocalization of the Ty-Tag and αRNG2 antibody with the N-terminal (Myc) or C-terminal (HA) tags performed by U-ExM with extruded conoid. Scale bar = 3 μm. Plot profile analysis of the signal. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(D)</b> Colocalization of the Ty-Tag with the N-terminal (Myc) or C-terminal (HA) tags performed by U-ExM with retracted conoid. Scale bar = 3 μm. Plot profile analysis of the signal. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p>2025-11-24T18:30:50ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003506.s003https://figshare.com/articles/figure/Additional_information_on_the_processed_forms_and_localization_of_the_RNG2_protein_/30697317CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306973172025-11-24T18:30:50Z |
| spellingShingle | Additional information on the processed forms and localization of the RNG2 protein. Romuald Haase (22339777) Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| status_str | publishedVersion |
| title | Additional information on the processed forms and localization of the RNG2 protein. |
| title_full | Additional information on the processed forms and localization of the RNG2 protein. |
| title_fullStr | Additional information on the processed forms and localization of the RNG2 protein. |
| title_full_unstemmed | Additional information on the processed forms and localization of the RNG2 protein. |
| title_short | Additional information on the processed forms and localization of the RNG2 protein. |
| title_sort | Additional information on the processed forms and localization of the RNG2 protein. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |