A Simple and Flexible Strategy for Single-Cell Proteomic Analysis Based on Protein Immobilization and Digestion Tube Reactor

Mass spectrometry-based single-cell proteomics (SCP) analysis has witnessed rapid development over the past 10 years. However, the current preprocessing methodologies face several challenges: multiple time-consuming steps, reliance on costly consumables and advanced instrumentation, and the necessit...

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Bibliographic Details
Main Author:	Wenjia Zhang (417706) (author)
Other Authors:	Lingxiao Weng (13267368) (author), Guoquan Yan (465897) (author), Wei Liu (20030) (author), Xuantang Wang (5977874) (author), Mingxia Gao (1290741) (author), Xiangmin Zhang (813476) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Cell Biology Genetics Physiology Biotechnology Immunology Developmental Biology Cancer Hematology Space Science witnessed rapid development past 10 years inner surface functionalization facilitates process automation cervical cancer cells addressing biological problems 7 cells (< 4018 protein groups relatively large volume additional transfer steps seamlessly integrates single digestion tube reactor 16 ), respectively cell protein immobilization deep scp analysis protein immobilization 16 ), picoliter volume consuming steps spidr ), cell sampling cell proteomics cell level widespread application spidr workflow specialized expertise remains free proteomic heterogeneity popular nanoliter n </ multiple time microliter single microliter reactor low cost flexible strategy costly consumables based single advanced instrumentation
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A Simple and Flexible Strategy for Single-Cell Proteomic Analysis Based on Protein Immobilization and Digestion Tube Reactor

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