Reconstruction of the Fig 4E panel III.

Reconstruction of the Fig 4E panel III.

<div><p>The conoid is a dynamic, tubulin-based structure conserved across the Apicomplexa that undergoes extrusion during egress, gliding motility, and invasion in <i>Toxoplasma gondii</i>. This organelle traverses the apical polar ring (APR) in response to calcium waves and...

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Opis bibliograficzny
1. autor:	Romuald Haase (22339777) (author)
Kolejni autorzy:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Wydane:	2025
Hasła przedmiotowe:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Reconstruction of the Fig 4E panel II.
od: Romuald Haase (22339777)
Wydane: (2025)

Reconstruction of the Fig 4E panel I.
od: Romuald Haase (22339777)
Wydane: (2025)

Model RNG2 as a molecular tether for conoid apical anchorage.
od: Romuald Haase (22339777)
Wydane: (2025)

RNG2 is functional only as intact, full-length protein.
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Investigation of RNG2 subdomain functions.
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Phenotypic consequences of the conoid detachment.
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Wydane: (2025)

RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
od: Romuald Haase (22339777)
Wydane: (2025)

RNG2 spans between the APR and the conoid, forming 22 tethers.
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Wydane: (2025)

Biochemical investigation of wild-type and processed RNG2.
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Wydane: (2025)

<i>Toxoplasma gondii</i> tachyzoite cell organization.
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Wydane: (2025)

Source Numerical Data of this study.
od: Romuald Haase (22339777)
Wydane: (2025)

Additional information on antibodies and primers used in this study.
od: Romuald Haase (22339777)
Wydane: (2025)

Additional information on the RNG2 variants expressed in UPRT locus.
od: Romuald Haase (22339777)
Wydane: (2025)

Additional information on the cryo-electron tomography performed on RNG2-mAID strain.
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Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
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Additional information on the processed forms and localization of the RNG2 protein.
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Multiple epitopes-tagging across RNG2.
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Additional information of the RNG2 purification from insect cells.
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A. Mouse transcriptome non-negative matrix factorization component weights across all time points and conditions. Larger weight values indicate components with upregulated genes dominating. B. Gene set enrichment plot for selected features from each component in panel A. Components 1, 4, and 5 did not exhibit enrichment in these broad terms. C. Number of genes in enriched gene ontology terms related to immunity, signaling, and transcription for each time point and condition. D. Time course transcriptional profiles for mouse genes characteristic to component 3. E. PARFA-seq validation by quantitative PCR and gene expression changes during immortalized bone marrow-derived macrophage infection by <i>T. gondii</i> assessed by quantitative PCR.
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Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
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Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
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A. <i>T. gondii</i> gene group changes throughout the time course, expressed as log<sub>2</sub>(fold change) relative to pre-infection tachyzoites (t = 0). Grey area depicts the 95% confidence interval. n value refers to number of genes detected in each group. B. Gene set enrichment plot for most enriched terms from <i>T. gondii</i> transcriptome features at 15 min to 120 min. Five most significant terms are displayed for each database. C and D. Transcriptional profiles of top five upregulated and downregulated <i>T. gondii</i> transcription factors, respectively.
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Wydane: (2025)

A. Library preparation strategy: poly-A(+) RNAs are captured by complementarity to reverse transcription primer, then second strand is synthesized using random hexamers and handles for sequencing are attached through two rounds of PCR. B. Mapped read distribution by species and by biotype. C and D. Principal component analysis of mouse and <i>T. gondii</i> transcriptomes, respectively. E and F. Volcano plots of Live <i>Toxoplasma</i>-infected mouse and <i>T. gondii</i> time points, respectively. To calculate Log<sub>2</sub>(Fold change), every timepoint is compared to corresponding samples (either macrophage or <i>Toxoplasma</i>) before infection (t = 0 min). Most significant and most upregulated transcripts are labelled. Number of significantly (p ≤ 0.05) downregulated and upregulated genes for each time point is given in blue and red, respectively. In F, 24-hour time point, red dots mark ROP
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Wydane: (2025)

A. Experimental scheme of infection time course and downstream workflow; B. Optimization of tachyzoite concentration to achieve adequate infection rates, with a threshold set at 50% infection efficiency. Error bars represent ± 1 standard deviation. Corresponding MOI values are given for each condition; C. Infection time course micrograph merged from brightfield and green channels. <i>T. gondii</i> exhibits green fluorescence due to stable GFP expression in the strain used. Percentage at the top left indicates infection efficiency (± 1 standard deviation) for each time point.
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Wydane: (2025)

Summary of all significant gene enrichment terms for every condition (as combination of sample type and timepoint).
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Wydane: (2025)

Oligonucleotides used in this study.
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Summary of the general phenotype of SH3 domain-containing gene mutants.
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SH3 domain-containing genes and the subcellular localization of their respective products in <i>F. graminearum.</i>
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The IDRs in SH3 domain-containing proteins are important for the fusion of cytoplasmic protein condensates.
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SH3 domain-containing proteins exhibit features of phase separation in <i>F. graminearum.</i>
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Effects of <i>FgHOF1</i> deletion on cytoskeletal and nuclear distribution.
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Role of SH3 domain-containing proteins on deoxynivalenol (DON) production in <i>F. graminearum.</i>
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Effect of SH3 domain gene deletions on the pathogenicity of <i>F. graminearum.</i>
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