Hybrid Iterating-Averaging Low Photon Budget Gabor Holographic Microscopy

Hybrid Iterating-Averaging Low Photon Budget Gabor Holographic Microscopy

Achieving high-contrast, label-free imaging with minimal impact on live cell culture behavior remains a primary challenge in quantitative phase imaging (QPI). By enabling imaging under low illumination intensities (low photon budget, LPB), it is possible to minimize cell photostimulation, phototoxic...

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Bibliographic Details
Main Author: Mikolaj Rogalski (13931940) (author)
Other Authors: Piotr Arcab (13931943) (author), Emilia Wdowiak (18846937) (author), José Ángel Picazo-Bueno (20543410) (author), Vicente Micó (20543413) (author), Michal Józwik (20543416) (author), Maciej Trusiak (3958637) (author)
Published: 2025
Subjects:
Medicine
Cell Biology
Biotechnology
Space Science
Biological Sciences not elsewhere classified
Physical Sciences not elsewhere classified
Information Systems not elsewhere classified
potentially transforming biomedical
line holographic microscopy
fidelity phase images
dynamic sperm cells
optically thin samples
dynamic biological samples
twin image disturbance
confirmed iga ’
advancements make iga
noise holograms even
qpi technique known
quantitative phase imaging
low photon budget
minimize cell photostimulation
iterative gabor averaging
high photon budgets
camera shot noise
low illumination intensities
iga algorithm achieves
image samples
shot noise
low illumination
iterative process
frame averaging
quantification noise
noise conditions
qpi due
qpi ).
speed imaging
enabling imaging
supporting long
speed observations
simultaneous minimization
simulations demonstrate
reconstructs high
primary challenge
powerful tool
novel approach
minimal impact
lpb data
lpb ),
light settings
hybrid iterating
high levels
environmental applications
effectively suppress
critical challenge
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