Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia
<p>CD19-NKCEs efficiently promote NK-cell antileukemia activity in a transplantation setting. <b>A,</b> Evaluation of NKp46, NKp30, CD16, perforin, and granzyme B expression in NK cells from transplanted patients (Post1–3M; <i>n</i> = 12) and healthy donors (HD; <i&g...
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2025
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| 總結: | <p>CD19-NKCEs efficiently promote NK-cell antileukemia activity in a transplantation setting. <b>A,</b> Evaluation of NKp46, NKp30, CD16, perforin, and granzyme B expression in NK cells from transplanted patients (Post1–3M; <i>n</i> = 12) and healthy donors (HD; <i>n</i> = 5–11). <b>B,</b> Representative experiment of cytototoxicity (7AAD/AnnV staining). MHH-CALL-4 or ALL#06 primary leukemia target cells were cultured either alone (only target) or with resting NK cells from a transplanted patient (3 months after haplo-HSCT) and the indicated NKCEs (10<sup>0</sup> μg/mL). Numbers indicate the percentage of cells in each quadrant. Percent of specific lysis (7AAD/AnnV staining; <b>C</b>) and CD107a degranulation of resting NK cells (<b>D</b>) from transplanted patients against MHH-CALL-4 (left) or primary leukemia blasts (ALL#04 and ALL#06; right) in the presence of the indicated NKCE at 10<sup>0</sup> μg/mL. Pooled data obtained with primary leukemia blasts are shown. Results from 5–8 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.01; ***, <i>P</i> ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.</p> |
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