Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia
<p>CD19-NKCEs efficiently promote NK-cell antileukemia activity in a transplantation setting. <b>A,</b> Evaluation of NKp46, NKp30, CD16, perforin, and granzyme B expression in NK cells from transplanted patients (Post1–3M; <i>n</i> = 12) and healthy donors (HD; <i&g...
Сохранить в:
| Главный автор: | |
|---|---|
| Другие авторы: | , , , , , , , , , , , , , |
| Опубликовано: |
2025
|
| Предметы: | |
| Метки: |
Добавить метку
Нет меток, Требуется 1-ая метка записи!
|
| _version_ | 1849927631567323136 |
|---|---|
| author | Natalia Colomar-Carando (14611340) |
| author2 | Laurent Gauthier (15129623) Pietro Merli (15129626) Fabrizio Loiacono (5306981) Paolo Canevali (332990) Michela Falco (368897) Federica Galaverna (8825531) Benjamin Rossi (15129629) Frédéric Bosco (15129632) Mélody Caratini (15129635) Maria Cristina Mingari (8409327) Franco Locatelli (15127797) Eric Vivier (48284) Raffaella Meazza (7179467) Daniela Pende (15129638) |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Natalia Colomar-Carando (14611340) Laurent Gauthier (15129623) Pietro Merli (15129626) Fabrizio Loiacono (5306981) Paolo Canevali (332990) Michela Falco (368897) Federica Galaverna (8825531) Benjamin Rossi (15129629) Frédéric Bosco (15129632) Mélody Caratini (15129635) Maria Cristina Mingari (8409327) Franco Locatelli (15127797) Eric Vivier (48284) Raffaella Meazza (7179467) Daniela Pende (15129638) |
| author_role | author |
| dc.creator.none.fl_str_mv | Natalia Colomar-Carando (14611340) Laurent Gauthier (15129623) Pietro Merli (15129626) Fabrizio Loiacono (5306981) Paolo Canevali (332990) Michela Falco (368897) Federica Galaverna (8825531) Benjamin Rossi (15129629) Frédéric Bosco (15129632) Mélody Caratini (15129635) Maria Cristina Mingari (8409327) Franco Locatelli (15127797) Eric Vivier (48284) Raffaella Meazza (7179467) Daniela Pende (15129638) |
| dc.date.none.fl_str_mv | 2025-11-25T13:42:39Z |
| dc.identifier.none.fl_str_mv | 10.1158/2326-6066.30710194 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Figure_4_from_Exploiting_Natural_Killer_Cell_Engagers_to_Control_Pediatric_B-cell_Precursor_Acute_Lymphoblastic_Leukemia/30710194 |
| dc.rights.none.fl_str_mv | CC BY info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cancer Immuno-oncology Hematological Cancers Leukemias Immunology NK cells Immunotherapy Stem cell transplantation |
| dc.title.none.fl_str_mv | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>CD19-NKCEs efficiently promote NK-cell antileukemia activity in a transplantation setting. <b>A,</b> Evaluation of NKp46, NKp30, CD16, perforin, and granzyme B expression in NK cells from transplanted patients (Post1–3M; <i>n</i> = 12) and healthy donors (HD; <i>n</i> = 5–11). <b>B,</b> Representative experiment of cytototoxicity (7AAD/AnnV staining). MHH-CALL-4 or ALL#06 primary leukemia target cells were cultured either alone (only target) or with resting NK cells from a transplanted patient (3 months after haplo-HSCT) and the indicated NKCEs (10<sup>0</sup> μg/mL). Numbers indicate the percentage of cells in each quadrant. Percent of specific lysis (7AAD/AnnV staining; <b>C</b>) and CD107a degranulation of resting NK cells (<b>D</b>) from transplanted patients against MHH-CALL-4 (left) or primary leukemia blasts (ALL#04 and ALL#06; right) in the presence of the indicated NKCE at 10<sup>0</sup> μg/mL. Pooled data obtained with primary leukemia blasts are shown. Results from 5–8 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.01; ***, <i>P</i> ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_51edda2d78b5d2883c32afc479a13056 |
| identifier_str_mv | 10.1158/2326-6066.30710194 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30710194 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY |
| spelling | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic LeukemiaNatalia Colomar-Carando (14611340)Laurent Gauthier (15129623)Pietro Merli (15129626)Fabrizio Loiacono (5306981)Paolo Canevali (332990)Michela Falco (368897)Federica Galaverna (8825531)Benjamin Rossi (15129629)Frédéric Bosco (15129632)Mélody Caratini (15129635)Maria Cristina Mingari (8409327)Franco Locatelli (15127797)Eric Vivier (48284)Raffaella Meazza (7179467)Daniela Pende (15129638)CancerImmuno-oncologyHematological CancersLeukemiasImmunologyNK cellsImmunotherapyStem cell transplantation<p>CD19-NKCEs efficiently promote NK-cell antileukemia activity in a transplantation setting. <b>A,</b> Evaluation of NKp46, NKp30, CD16, perforin, and granzyme B expression in NK cells from transplanted patients (Post1–3M; <i>n</i> = 12) and healthy donors (HD; <i>n</i> = 5–11). <b>B,</b> Representative experiment of cytototoxicity (7AAD/AnnV staining). MHH-CALL-4 or ALL#06 primary leukemia target cells were cultured either alone (only target) or with resting NK cells from a transplanted patient (3 months after haplo-HSCT) and the indicated NKCEs (10<sup>0</sup> μg/mL). Numbers indicate the percentage of cells in each quadrant. Percent of specific lysis (7AAD/AnnV staining; <b>C</b>) and CD107a degranulation of resting NK cells (<b>D</b>) from transplanted patients against MHH-CALL-4 (left) or primary leukemia blasts (ALL#04 and ALL#06; right) in the presence of the indicated NKCE at 10<sup>0</sup> μg/mL. Pooled data obtained with primary leukemia blasts are shown. Results from 5–8 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.01; ***, <i>P</i> ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.</p>2025-11-25T13:42:39ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1158/2326-6066.30710194https://figshare.com/articles/figure/Figure_4_from_Exploiting_Natural_Killer_Cell_Engagers_to_Control_Pediatric_B-cell_Precursor_Acute_Lymphoblastic_Leukemia/30710194CC BYinfo:eu-repo/semantics/openAccessoai:figshare.com:article/307101942025-11-25T13:42:39Z |
| spellingShingle | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia Natalia Colomar-Carando (14611340) Cancer Immuno-oncology Hematological Cancers Leukemias Immunology NK cells Immunotherapy Stem cell transplantation |
| status_str | publishedVersion |
| title | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| title_full | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| title_fullStr | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| title_full_unstemmed | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| title_short | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| title_sort | Figure 4 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia |
| topic | Cancer Immuno-oncology Hematological Cancers Leukemias Immunology NK cells Immunotherapy Stem cell transplantation |