Workflow of the flow cytometry experiment and the software-based analysis.

<p>(1) Raw data were generated by flow cytometry. (2) FCS files were imported into the Cytolution software. Initial quality control included panel check, compensation data check, automatic control of the time channel and removal of cell debris and cell aggregates. In this step, the user is abl...

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Bibliographic Details
Main Author:	Verena Fischer (3857632) (author)
Other Authors:	Anita Ignatius (142960) (author), Katharina Schmidt-Bleek (283466) (author), Georg Duda (283456) (author), Melanie Haffner-Luntzer (504724) (author)
Published:	2025
Subjects:	Cell Biology Genetics Physiology Ecology Immunology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified surface marker expression particular granulocyte subpopulation immune cell subtypes immune cell populations adaptive immune response single threshold based flow cytometry software using artificial intelligence particularly well documented binary &# 8220 4 subclusters ), based clustering software impaired fracture healing early fracture hematoma based software fracture hematoma well established delayed healing assessed using &# 8221 several subclusters 6 subclusters xlink "> unbiased discrimination specific role significant reduction results suggest recent advances previous studies powerful tool polarization status nuanced understanding macrophage subpopulations increased abundance day 1 bone regeneration bone marrow also altered
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Workflow of the flow cytometry experiment and the software-based analysis.

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