Surface-Engineered Filtration Device with Silver Nanoparticle–Silane Modifications for Enhanced Intracellular Delivery and Cell Transfection

Despite promising therapeutic outcomes from cellular and gene therapies (CGTs), their widespread clinical application remains limited by the high costs and technical complexities of conventional transfection methods. We present an optimized filtration-driven membrane poration platform that addresses...

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Autore principale:	Chuan-Hao Kuo (22058472) (author)
Altri autori:	Sheng-Han Yang (22058475) (author), Zhen-Kai Lin (22058478) (author), Woei-Cherng Shyu (308328) (author), Long-Bin Jeng (499919) (author), Chih-Sheng Chiang (14977287) (author)
Pubblicazione:	2025
Soggetti:	Biophysics Biochemistry Medicine Cell Biology Molecular Biology Biotechnology Space Science Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified scale screening applications optimizing membrane disruption functional plasmid dna enhanced intracellular delivery diverse cargo types derived stem cells addresses key limitations silver ion effects introduces hydrophobic properties improve cell recovery engineered filtration device conventional transfection methods optimized system demonstrates conventional methods optimized filtration system achieves silver nanoparticles hydrophobic surfaces cell passage optimized configuration yield using unmodified filters technical complexities silane coating physical interactions limited laboratories high costs gene therapies filter paper cgts ), adscs ). accessible alternative
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Surface-Engineered Filtration Device with Silver Nanoparticle–Silane Modifications for Enhanced Intracellular Delivery and Cell Transfection

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