Purification of IpaC NTD (residues 1-100) by nickel affinity chromatography.

<p>Lanes: 1 molecular weight marker, 2 before IPTG induction, 3 after IPTG induction, 4-6 supernatants of pellets washes, 7 flowthrough, 8-9 washes, 10 molecular weight marker, 11 wash, 12-14 elutions, 15 column strip with 0.5 M EDTA.</p>

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Bibliografiske detaljer
Hovedforfatter:	Jacob M. Kroh (22238110) (author)
Andre forfattere:	Amritangshu Chakravarty (22238113) (author), Supratim Dey (22238116) (author), Roberto N. De Guzman (22238119) (author)
Udgivet:	2025
Fag:	Biophysics Biochemistry Microbiology Cell Biology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified random coil conformation predominantly alpha helical many bacterial pathogens cause infectious diseases alphafold predictions suggested alpha helical bundles 2d nmr show lack tertiary structures alpha helical proteins two membrane proteins div >< p t3ss translocases ipac secondary structures protein structures dimensional structures thermal denaturation t3ss consists significant amount shigella </ salmonella </ relative sizes minor translocases initiate infection human cells host membrane highest level circular dichroism cd spectroscopy burkholderia </ bacteria use atomic structure
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Purification of IpaC NTD (residues 1-100) by nickel affinity chromatography.

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