Strategy to clone level 0 modules using the MoClo Toolkit (Addgene, kit #1000000044) universal level 0 acceptor plasmid (pAGM9121).

(A) Schematic representation of the level 0 acceptor plasmid and the cleavage site of the BpiI enzyme; (B) Schematic representations of different approaches to prepare the FOI for cloning: (B1) Synthesize the DNA sequence of interest; (B2) Design primer...

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Autor principal:	Inês M. Luís (3210918) (author)
Altres autors:	Mariana Parada (21672880) (author), João B. Vicente (9562318) (author), Isabel A. Abreu (2533357) (author)
Publicat:	2025
Matèries:	Biophysics Biochemistry Molecular Biology Neuroscience Biotechnology Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified α reporter gene human ace2 using functional human ace2 div >< p compatible pet vector achieve high levels soluble protein fraction escherichia coli </ soluble protein expression golden gate cloning level 0 modules protein expression lacz </ tools facilitate successfully produced successful purification step instructions peptidase domain new tool multiple constructs modular system moclo toolkit includes step golden gate first report final plasmid express constructs efficient pipelines comprehensive guide addgene kit >, validated >), provides 1000000044 ).
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Strategy to clone level 0 modules using the MoClo Toolkit (Addgene, kit #1000000044) universal level 0 acceptor plasmid (pAGM9121).

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