Figure S1 from T-cell Receptor Therapy Targeting Mutant Capicua Transcriptional Repressor in Experimental Gliomas

<p>Supplementary Figure 1: T cell responses in A2.DR1 and DR4 mice after peptide vaccination with potential shared neoepitopes. A. Predicted binding affinity for selected amino acid alterations for HLA-A*0201 determined by NetMHC 4.0. B, C. Quantification of IFN-γ ELISpot using cells isolated...

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Egile nagusia: Michael Kilian (16697663) (author)
Beste egile batzuk: Mirco Friedrich (3721555) (author), Khwab Sanghvi (16697666) (author), Edward Green (3822586) (author), Stefan Pusch (14934437) (author), Daisuke Kawauchi (15115187) (author), Martin Löwer (133159) (author), Jana K. Sonner (16697669) (author), Christopher Krämer (16697672) (author), Julia Zaman (16697675) (author), Stefanie Jung (554730) (author), Michael O. Breckwoldt (16697678) (author), Gerald Willimsky (16697681) (author), Stefan B. Eichmüller (16697684) (author), Andreas von Deimling (15037904) (author), Wolfgang Wick (15018168) (author), Felix Sahm (15097382) (author), Michael Platten (15018165) (author), Lukas Bunse (16697687) (author)
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Gaia:<p>Supplementary Figure 1: T cell responses in A2.DR1 and DR4 mice after peptide vaccination with potential shared neoepitopes. A. Predicted binding affinity for selected amino acid alterations for HLA-A*0201 determined by NetMHC 4.0. B, C. Quantification of IFN-γ ELISpot using cells isolated from lymph nodes from vaccinated A2.DR1 mice restimulated with the indicated peptide. D. ELISpot of splenocytes from 27-mer CICR215W-vaccinated mice restimulated for 48 h with the indicated 15-mer (see also Supplementary Table 1). B, C, D Data are represented as mean {plus minus} SEM, statistical significance was determined by unpaired two-tailed Student's t-test. E. Intracellular IFN-γ flow cytometric analyses after in vitro re-stimulation of splenocytes from 27-mer CICR215W-vaccinated DR4 mice gated on single, living, and CD3+ cells.</p>