Selected Ion Extraction of Peptides with Heavy Isotopes and Hydrogen Loss Reduces the Type II Error in Plasma Proteomics

Naturally occurring peptides display a wide mass distribution after ionization due to the presence of heavy isotopes of C, H, N, O, and S and hydrogen loss. There is a crucial need for sensitive methods that collect as much information as possible about all plasma peptide forms. Statistical analysis...

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Bibliographic Details
Main Author:	Jaimie Dufresne (4541728) (author)
Other Authors:	Zhuo Zhen Chen (10841817) (author), Pallvi Sehajpal (20495328) (author), Peter Bowden (744696) (author), Ja-An Ho (20495331) (author), Cheng-Chih Richard Hsu (20495334) (author), John G. Marshall (744697) (author)
Published:	2025
Subjects:	Biochemistry Pharmacology Immunology Infectious Diseases Chemical Sciences not elsewhere classified wide mass distribution selected ion extraction quaternary amine resin orbital ion trap increase observation frequencies delta mass distribution comparative proteomics studies human plasma samples 077 peptide sequences plasma peptide forms hydrogen loss resulted hydrogen loss reduces peptide sequences identified 29 blank lc known plasma proteins 963 proteins (< monoisotopic peak alone gaussian heavy isotopes ms control spectra 1 da around type ii error ≥ 3 ). hydrogen loss heavy isotopes plasma proteins peptide precursors peptide counts ≥ 3 monoisotopic peak gaussian peaks ms spectra fragment spectra 5 da 1 da peptides identified peptides (< resulting proteins oit ). − 3 − 1 true positives statistical analysis sensitive methods q </ much information ionization due high confidence experimental ms crucial need alb ),
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Selected Ion Extraction of Peptides with Heavy Isotopes and Hydrogen Loss Reduces the Type II Error in Plasma Proteomics

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