Additional information on the RNG2 variants expressed in UPRT locus.

(A) IFA localization and plaque assay assessment of the first round of RNG2 variants. Cell marker: IMC1. Scale bar = 2 μm. (B) IFA localization and plaque assay assessment of the second round of the RNG2 variant. Cell marker: GAP45. Scale bar = 2 μm <...

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Hlavní autor:	Romuald Haase (22339777) (author)
Další autoři:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Vydáno:	2025
Témata:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Shrnutí:	<p><b>(A)</b> IFA localization and plaque assay assessment of the first round of RNG2 variants. Cell marker: IMC1. Scale bar = 2 μm. <b>(B)</b> IFA localization and plaque assay assessment of the second round of the RNG2 variant. Cell marker: GAP45. Scale bar = 2 μm <b>(C)</b> IFA localization and plaque assay assessment of the processed forms observed in insect cells and expressed in the UPRT locus. Cell marker: GAP45. Scale bar = 2 μm.</p> <p>(TIFF)</p>

Additional information on the RNG2 variants expressed in UPRT locus.

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