Purification of hGPER and estradiol binding activity of purified protein.

<p>(A) Protein bands were detected using CBBR staining or immunostaining with an anti-His tag antibody (α-His). An arrowhead indicates hGPER. (B) The binding activity of hGPER to [2,4,6,7 <sup>3</sup>H]-estradiol was measured at different concentrations of digitonin (0, 0.01 and 0....

Полное описание

Сохранить в:

Библиографические подробности
Главный автор:	Shakhawat Hossain (3857050) (author)
Другие авторы:	Md. Forhad Hossain (13732290) (author), Md. Sohanur Rahman Sohan (22279202) (author), Yuki Omori (6986495) (author), Mohammad Tohidul Amin (19286549) (author), Toshinobu Tokumoto (61792) (author)
Опубликовано:	2025
Предметы:	Biophysics Biochemistry Genetics Molecular Biology Neuroscience Physiology Pharmacology Biotechnology Immunology Cancer Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified screening potential compounds pichia pastoris </ optimizing codon usage graphene quantum dots membrane estrogen receptors div >< p coupled estrogen receptor expression system using successfully expressed hgper gqds )</ p homogeneous assay established homogeneous assay nongenomic estrogen successfully established gqds coupled binding assay subsequently established stimulating effects recombinant protein reaction mixture radiolabeled steroid protein via promising candidate nongenomic actions methylotrophic yeast key mediator hgper ligands gpcr family g protein free estradiol drug discovery column chromatography binding activity 76 nm 520 nm
Метки:	Добавить метку Нет меток, Требуется 1-ая метка записи!

Purification of hGPER and estradiol binding activity of purified protein.

Схожие документы