Simultaneous Profiling of Multiple Phosphorylated Metabolites in Typical Biological Matrices via Ion-Pair Reversed-Phase Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry

Simultaneous analysis of multiple phosphorylated metabolites (phosphorylated metabolome) in biological samples is vital to reveal their physiological and pathophysiological functions, which is extremely challenging due to their low abundance in some biological matrices, high hydrophilicity, and poor...

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Bibliographic Details
Main Author:	Huan Wang (109286) (author)
Other Authors:	Qinsheng Chen (7304777) (author), Yuanpeng Wu (2590396) (author), Li Xu (108702) (author), Qi Wang (22418) (author), Qingyu Hu (12399292) (author), Huiru Tang (115310) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Genetics Molecular Biology Physiology Biotechnology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified tested analytes especially poor chromatographic behavior performance liquid chromatography hybrid surface technology extremely challenging due various phosphorylated metabolites phosphorylated metabolite composition multiple phosphorylated metabolites plasma ), cells including phosphorylated sugars r </ sub phosphorylated metabolome including tissue >< sub tailing factor simultaneous profiling phase ultrahigh pathophysiological functions pair reversed new method n </ low abundance high hydrophilicity capacity factor biological samples biological matrices based cofactors amino acids 238 fmol 2 ), 09 min
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Simultaneous Profiling of Multiple Phosphorylated Metabolites in Typical Biological Matrices via Ion-Pair Reversed-Phase Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry

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