Gel electrophoresis analysis of I-SceI digested DNA fragments.

A): I-SceI, NdeI and SalI digestion maps of the E. coli chromosomal region surrounding the DSB site. The restriction sites and the distance between them are marked with blue vertical arrows and numbers (in kb), respectively. The DSB site is marked by an...

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Bibliographic Details
Main Author:	Tahirah Yasmin (5130611) (author)
Other Authors:	Benura Azeroglu (1783102) (author), Fares Osam Yanez-Cuna (19838098) (author), Sally Jones (7186193) (author), Patrick Yizhi Cai (19838101) (author), David R. F. Leach (8212269) (author)
Published:	2024
Subjects:	Biophysics Biochemistry Genetics Molecular Biology Biotechnology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified would otherwise dominate study reveals ways direct visualization provides 6 mb genome specific dna samples specific dna molecules dna molecules becomes complex dna molecules strand break repair gel electrophoresis followed div >< p branched dna molecules agarose gels subjected recombination intermediates may dna molecules dna replication dna double agarose embedded recombination intermediates key intermediates vivo </ various single using β unwanted non technical challenges structural details second round sample needs molecule approach molecule analyses molecular weight molecular pathways microscopic examination mechanisms underlying important factor effectively analysed e </ could obscure constitutes less consider since complementary single coli </ branched structures agarase digestion 40 kb
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Gel electrophoresis analysis of I-SceI digested DNA fragments.

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