Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
<p>Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a signif...
Kaydedildi:
| Yazar: | |
|---|---|
| Diğer Yazarlar: | , , , |
| Baskı/Yayın Bilgisi: |
2025
|
| Konular: | |
| Etiketler: |
Etiketle
Etiket eklenmemiş, İlk siz ekleyin!
|
| Özet: | <p>Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.</p> |
|---|